曲古菌素A对结肠癌Lovo细胞增殖抑制的研究

目的:研究组蛋白去乙酰化酶抑制剂曲古菌素A（TSA）对结肠癌Lovo细胞增殖活性的抑制作用。方法:采用不同浓度的TSA处理结肠癌Lovo细胞。MTT法检测药物作用前后的细胞增殖情况。流式细胞仪检测TSA处理前后细胞周期的变化。结果:TSA在500ng/mL浓度以上才能明显抑制结肠癌Lovo细胞的增殖，抑制率由第2天至第5天显著增加（57.21%至82.76%）细胞周期检测发现20ng/mL TSA即可导致细胞G1期阻滞，但没有诱导明显的细胞凋亡;≥100ng/mL TSA可诱导明显的细胞凋亡。结论:TSA可以抑制体外结肠癌Lovo细胞的生长，可能通过细胞周期G1期阻滞及诱导细胞凋亡发挥抑癌作用。TSA可能是结肠癌治疗的潜在靶点。

Effects of trichostatin A on the growth of colon cancer cells

Abstract:Objective:To investigate whether trichostatin A (TSA) possesses antitumor activity against human colon cancer cells. Methods :Human colon cancer cell line Lovo was treated with different concentrations of TSA. The growth of Lovo cells were observed by MTT assay before and after TSA treatment. The cell cycle of Lovo cells was analyzed by flow cytometry.Results:Trichostatin A significantly inhibited the proliferation of colon cancer cells at 500 ng/ml. The inhibition rate increased sharply from day 2 to day 5 (57.21% to 82.76%). 20 ng/ml TSA treatment could induce cell cycle arrest at the G1 phase, but with no apparent increase in apoptotic cells. ≥100 ng/ml TSA could induce significant apoptosis as shown by flow cytometry.Conclusions:The results indicate that TSA is able to inhibit colon cancer cell growth in vitro, possibly through G1 cell cycle arrest and induction of apoptosis. This study suggests that TSA may be a potential therapeutic agent for the target treatment of colon cancer.