BXSB小鼠肝、肾、脾脏器中C－myc和N－ras原癌基因的表达

用同位素标记ｃＤＮＡ探针的斑点杂交方法观察了ＢＸＳＢ小鼠肝、肾、脾脏器中Ｃ－ｍｙｃ和Ｎ－ｒａｓ原癌基因的表达情况。结果表明ＳＬＥ发病较重的４～６月龄ＢＸＳＢ雄鼠脾脏和肾脏均表达Ｃ－ｍｙｃｍＲＮＡ，肝脏未见表达。ＳＬＥ发病较轻的４～６周龄ＢＸＳＢ雄鼠仅发现脾脏表达Ｃ－ｍｙｃｍＲＮＡ。ＢＸＳＢ小鼠肝、肾、脾各脏器均无Ｎ－ｒａｓｍＲＮＡ表达。本研究结果提示Ｃ－ｍｙｃ基因可能参与ＢＸＳＢ小鼠淋巴细胞异常活化和狼疮性肾炎的发生。

Expression of C-myc and N-ras oncogenes in Liver,Kidney and Spleen of BXSB Mice

BXSB mice is one of the experimental mouse models for human systemic lupus erythematosus. By means of dot blot hybridization with isotope labeled probe,we detected the expression of C-myc and N-ras oncogene in liver, kidney and spleen of BXSB mice. High amount of C-myc mRNA was found both in spleen of 4～6 weeks old (pre disease) and 4 ～ 6 months old (clinically active) male BXSB autoimmune mice. C-myc mRNA was also found in the kidney of 4 ～6 moths old male BXSB mice. N-ras mRNA was not found in liver,kidney and spleen of BXSB mice. This result indicates that C-myc gene may play a role both in abnormal activation of lymphocyte and lupus nephritis in BXSB mice.