| DDPH在低氧内皮细胞条件培养液促肺动脉平滑肌细胞增殖中对c-fos和c-myc表达的影响 |
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| 引用本文： | 陈娟,周洁,谢秋红,段秋红,从容,王西明,冯友梅. DDPH在低氧内皮细胞条件培养液促肺动脉平滑肌细胞增殖中对c-fos和c-myc表达的影响[J]. 医学分子生物学杂志, 2005, 2(4): 250-253 |
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| 作者姓名： | 陈娟 周洁 谢秋红 段秋红 从容 王西明 冯友梅 |
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| 作者单位： | 华中科技大学同济医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院生物化学与分子生物学系,武汉市,430030 |
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| 基金项目： | 国家自然科学基金(No．39270285)~~ |
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| 摘 要： | 目的在细胞水平研究1-(2,6-二甲基苯氧基)-2-(3,4-二甲氧基苯乙胺基)丙烷盐酸盐(DDPH)在低氧内皮细胞条件培养基(HECCM)促肺动脉平滑肌细胞(PASMC)的增殖过程中对c-fos和c-myc表达的影响。方法采用MTT比色法作为HECCM促PASMC的增殖指标,采用核酸斑点杂交法检测c-fos与c-myc mRNA的相对表达量,同时观察DDPH对它们的影响。结果低氧可刺激内皮细胞产生某些因子,经旁分泌作用促进PASMC的c-fos和c-myc基因转录以及促使PASMC增殖。DDPH能显著抑制HECCM促PASMC的增殖作用以及下调c-fos和c-myc mRNA的表达量。结论 DDPH能显著抑制HECCM促PASMC的增殖作用,其作用机制可能是通过下调C-fos和C-myc mRNA的表达量来实现的。
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| 关 键 词： | 低氧 肺动脉平滑肌细胞 c-fos c-myc DDPH |
| 修稿时间： | 2005-04-19 |
Effect of DDPH on the Expression of c-fos and c-myc in the Pulmonary Arterial Smooth Muscule Cell Proliferation Induced by Hypoxic Endothelial Cell Conditioned Medium |
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| CHEN Juan,ZHOU Jie,XIE Qiuhong,DUAN Qiuhong,CONG Rong,FENG Youmei. Effect of DDPH on the Expression of c-fos and c-myc in the Pulmonary Arterial Smooth Muscule Cell Proliferation Induced by Hypoxic Endothelial Cell Conditioned Medium[J]. Journal of Medical Molecular Biology, 2005, 2(4): 250-253 |
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| Authors: | CHEN Juan ZHOU Jie XIE Qiuhong DUAN Qiuhong CONG Rong FENG Youmei |
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| Affiliation: | CHEN Juan,ZHOU Jie,XIE Qiuhong,DUAN Qiuhong,CONG Rong,FENG Youmei Department of Biochemistry and Molecular Biology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,China |
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| Abstract: | Objective This study examined the effect of DDPH on the expression of c-fos and c-myc oncogenes in the pulmonary arterial smooth muscle cell (PASMC) proliferation induced by hypoxic endothelial cell conditioned medium (HECCM). Method Cell culture, MTT colorimetry and dot blot methods were used to study the effects of HECCM on the PASMC proliferation and the effects of DDPH on the expression of c-fos and c-myc. Results The results indicated that HECCM induced expression of c-myc and c-fos oncogenes in the PASMCs. DDPH could obviously inhibit the proliferation of PASMC induced by HECCM and downregulate the expression of c-fox and c-myc mRNA. Conclusion It was suggested that the immediate early oncogene products, C-fos and C-myc, might play an important role in the proliferation of PASMC induced by HECCM. |
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| Keywords: | hypoxia pulmonary arterial smooth muscle cell c-myc c-fos DDPH |
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