不同品种地黄中毛蕊花糖苷的动态积累规律变化

目的:对大田生长期间不同品种地黄块根和叶片中的毛蕊花糖苷含量进行测定,研究道地产区不同品种地黄块根和叶片中毛蕊花糖苷的积累规律。方法:以栽培地黄85-5,北京1号,沁怀,金九和白选为材料,于2015年和2016年在河南温县大田栽培,以HPLC检测不同采收期不同品种地黄块根和叶片中的毛蕊花糖苷的含量。并以85-5为材料,于2014年进行遮阴处理,以HPLC检测遮阴后地黄块根和叶片中的毛蕊花糖苷含量。结果:不同品种地黄块根和叶片中毛蕊花糖苷含量有较大差异,块根中毛蕊花糖苷含量较高的2个品种是白选和沁怀,两年内毛蕊花糖苷含量变化分别为0.023%~0.620%和0.018%~0.796%;叶片中毛蕊花糖苷含量较高的品种为85-5和北京1号,毛蕊花糖苷含量变化分别为1.955%~5.968%和0.681%~5.941%。不同发育阶段的地黄叶片中,展开叶和衰老叶较幼嫩叶含有较高的毛蕊花糖苷。遮阴对地黄块根中毛蕊花糖苷含量有促进作用,对叶片中毛蕊花糖苷含量有抑制效应。结论:该研究为毛蕊花糖苷高含量地黄新品种的选育提供实验材料和依据,同时也为明确地黄毛蕊花糖苷积累变化的分子机制奠定基础。

Changes in Dynamic Accumulation of Acteoside from Different Rehmannia glutinosa Cultivars

Objective: To determine the content of acteoside in tuberous roots and leaves of different cultivars of Rehmannia glutinosa during their different growth periods at genuine producing areas, and investigate the changes in dynamic accumulation. Method: The acteoside contents from tuberous roots and leaves in 85-5, Beijing-1, Qinhuai, Jingjiu and Baixuan that were cultivated in 2015 and 2016 at Wenxian county were investigated by HPLC method during different harvest periods. Furthermore, acteoside content was detected in tuberous roots and leaves of 85-5 after shading treatments in 2014. Result: There were great differences of acteoside content in tuberous roots and leaves among the different cultivars of R. glutinosa. Acteoside contents were 0.023%-0.620% and 0.018%-0.796% in tuberous roots of Baixuan and Qinhuai respectively, which were higher than that of other cultivars. However, 85-5 and Beijing-1 had higher content of acteoside in leaves, ranging from 1.955%-5.968% and 0.681%-5.941%. The contents of acteoside were higher in unfolded leaves and senescing leaves than tender leaves. Shading improved its content in tuberous roots and restrained the accumulation of acteoside in leaves. Conclusion: The results provide the experimental materials and basis for breeding the high-acteoside-content R. glutinosa cultivars, and lay foundations for studying the regulation mechanisms of acteoside biosynthesis in R. glutinosa.