不同产地桔梗性状、浸出物、桔梗皂苷D含量及HPLC指纹图谱比较

目的:对国家资源普查收集的产自10个省市的58批桔梗样品进行性状鉴别,测定其浸出物、桔梗皂苷D的含量,建立HPLC指纹图谱并对其进行比较。方法:按照2015年版《中国药典》方法测定浸出物及桔梗皂苷D含量;采用高效液相-蒸发光散射检测法(HPLC-ELSD),梯度洗脱,建立不同产地桔梗样品HPLC指纹图谱。结果:58批桔梗样品中有2批表面色泽,7批大小,11批断面特征不符合2015年版《中国药典》规定;浸出物、桔梗皂苷D的含量的总不合格率为36.20%;建立了58批桔梗样品的特征指纹图谱,共找到17个共有峰;四川省桔梗样品浸出物、桔梗皂苷D含量及HPLC指纹图谱相似度3个方面均较高,重庆市、湖南省桔梗样品3个方面均较低;对58批不同产地桔梗进行了聚类分析及性状、浸出物、桔梗皂苷D含量和HPLC指纹图谱的比较研究,浸出物、桔梗皂苷D含量及HPLC指纹图谱相似度不成正向相关,但样品中浸出物含量以及各成分含量的差异与样品性状特征(皮部)具相关性。结论:所鉴别的性状特征,测定的浸出物、桔梗皂苷D含量以及建立的HPLC指纹图谱可为优质桔梗的筛选,新版《中国药典》标准的修订以及桔梗质量评价,提供更全面、真实、具代表性的参考依据。

Comparison of Traits, Root Extract, Platycodin D Content and HPLC Fingerprint of Platycodonis Radix from Different Habitats

Objective: To identify the traits of 58 batches of Platycodonis Radix samples from 10 provinces and cities, determine the content of root extract and platycodin D in the samples, establish and compare their High Performance Liquid Chromatography (HPLC) fingerprints. Method: The content of platycodin D and the root extract in Platycodonis Radix was determined according to the determination method in Chinese Pharmacopoeia. HPLC fingerprints of Platycodonis Radix were determined by High Performance Liquid Chromatography-Evaporative Lightscattering Detector (HPLC-ELSD) method and gradient elution flowing form. Result: In these 58 batches of sample, the surface color in 2 batches, size in 7 batches, and section characteristics in 11 batches did not comply with the requirements in pharmacopoeia. The content of platycodin D and root extract in 58 batches of Platycodonis Radix from 10 provinces or cities was determined, and the total failure rate of the root extract of Platycodonis Radix sample and platycodin D was 36.20%. A total of 17 common peaks were found in 58 batches of Platycodonis Radix. The content of extract and platycodin D, as well as HPLC fingerprints in Platycodonis Radix samples from Sichuan province showed high similarity, and these above three aspects showed low similarity in the samples from Chongqing and Hunan province. Cluster analysis and comparative study of the traits, root extract content, platycodin D content and HPLC fingerprints were performed on 58 batches of Platycodonis Radix from different habitats. The results showed that there was no positive correlation between the content of extract, platycodin D and HPLC fingerprint similarity, but the difference in content of extract and other components in the samples was related to the characteristics of the samples (skin). Conclusion: The identified traits, the determined content of platycodin D, root extract and the established HPLC fingerprints can provide a more comprehensive, authentic and representative reference for screening of high quality Platycodonis Radix, revision of the Chinese Pharmacopoeia and quality evaluation of Platycodonis Radix.