The effect of irbesartan on the expression of angiopoietin-like protein 2 in the kidneys of type2 diabetes rats

Objective To observe the effect of irbesartan on the expression of angiopoietin-like protein 2 (ANGPTL2) in the diabetic rats kidney and explore the underlying mechanism. Methods A total of sixty male SD rats were divided into normal control group (NC group, n=15) and experimental group (n=45) randomly. The experimental group was fed with high sugar-fat diet and given a low dose streptozocin（STZ 30 mg/kg）to establish type 2 diabetic model. Rats successfully induced diabetes were randomly divided into 2 groups: diabetes group (DM) and irbesartan group (DI). Weight, blood pressure, blood glucose, serum creatinine (Scr), blood urea nitrogen(BUN), 24 hour urinary albumin(UAL) and renal histomorphology were observed after drug intervention at the 4th, 8th and 12th weeks. The expression of ANGPTL2 in renal tissue were detected by immunohistochemistry, real-time PCR and Western blotting. Results The levels of Scr, BUN, TG, TC and UAL in group DM were higher than in group NC at the 4th, 8th and 12th week (all P＜0.05).Compared with that in group DM, above indexes were lower in group DI at the 4th, 8th and 12th week (all P＜0.05). The pathological changes of the kidney in group DM were more serious than that in group DI. The expression of ANGPTL2 in group DM was much higher than that in group NC at the 4th, 8th and 12th week (all P＜0.05), and irbesartan treatment inhibited the up-regulation of ANGPTL2 in group DI(all P＜0.05). Conclusion The expression of ANGPTL2 increases in T2DM rats kidney tissue with time and irbesartan can inhibit the up-regulation of ANGPTL2 in T2DM rats.