高效液相色谱法同时测定多维元素片中的维生素B2和叶酸

 目的 应用高效液相色谱建立多维元素片中维生素B₂和叶酸的同时测定方法。 方法 以0.5%氨水溶液为提取液，采用Alltima C₁₈色谱柱(4.6 mm×250 mm， 5 μm)，以50 mmol·L^-1磷酸二氢铵溶液（磷酸调至pH 3.0）-乙腈（85∶15）为流动相，流速0.5 mL·min^-1，检测波长为282 nm。结果 在本方法中，维生素B₂和叶酸分别在11.52~34.56和2.952~8.856 mg·L^-1内线性关系良好，相关系数（r）都大于0.999 9；2种成分和药品中其他成分以及强制降解产物之间均有良好的分离度；2种成分精密度实验的相对标准偏差（RSD）均小于2.0%；3个加样浓度的百分回收率都在97.95%和102.3%之间；常温下各种溶液中2种成分都在8 h内保持稳定。方法学比较显示：该方法和《美国药典》方法测定结果具有很好的一致性。结论 该方法准确、简单、快速，可作为多维元素片中维生素B₂和叶酸的同时测定方法。

Simultaneous Determination of Vitamin B2 and Folic Acid in Multivitamin with Multimineral Tablets by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of vitamin B2 and folic acid in Multivitamin with Multimineral Tablets.METHODS The samples were extracted with 0.5%(V/V) ammonia solution.An Alltima C18 column(4.6 mm×250 mm,5 μm) was used for the separation with 50 mmol·L-1 ammonium dihydrogen phosphate(adjusted pH to 3.0 with H3PO4) and acetonitrile(85∶15) as the mobile phase at the flow rate of 0.5 mL·min-1.The detection wavelength was 282 nm.RESULTS The method showed good linearity for vitamin B2 and folic acid with both correlation coefficients(r) greater than 0.999 9 in the ranges of 11.52-34.56 and 2.952-8.856 mg·L-1,respectively;the specificity study showed satisfactory resolutions between vitamin B2,folic acid,other ingredients and forced degradation products;the precisions were satisfactory with both relative standard deviations(RSD) lower than 2.0%;the spiked recoveriesy at three levels ranged from 97.95% to 102.3% for both compounds;the stability at ambient temperature was acceptable within 8 h for both compounds in combined standard solutions and analytical solutions.Method comparison indicated coincident results between this method and the USP method.CONCLUSION This method is an accurate,fast and simple method for the simultaneous determination of vitamin B2 and folic acid in Multivitamin with Multimineral Tablets.