巢式RT-PCR检胃癌患者外周血循环癌细胞的研究

目的　分析胃癌患者外周血中CK 2 0mRNA及CEAmRNA的异常表达及意义。方法　采用RT PCR方法检测 5 2例胃癌患者术前外周血中CK 2 0mRNA及CEAmRNA的表达。结果　胃癌患者外周血中CK 2 0mRNA、CEAmRNA阳性表达率分别为 42 .3 % (2 2 /5 2 )及 5 1.9% (2 7/5 2 ) ,CK 2 0mRNA和CEAmRNA两者中至少 1个基因为阳性的表达率为 63 .5 % (3 3 /5 2 )。胃癌患者外周血中CK 2 0mRNA表达与胃癌的远处转移有关 ,CEAmRNA表达、CK 2 0mRNA和CEAmRNA都表达、CK 2 0mR NA和CEAmRNA两者中至少 1个表达与胃癌的浸润深度、淋巴结转移和远处转移相关 (P <0 .0 5 )。结论　胃癌患者外周血CK 2 0和CEA的RT PCR检测有助于患者预后的判断 ;联合检测CK 2 0mRNA和CEAmRNA可增加外周血循环癌细胞检测的敏感性

Detection of the Cancer Cells by RT-PCR in Peripheral Blood of Patients with Gastric Cancer

Objective To investigate the expression of CK20 mRNA and CEA mRNA in peripheral blood of gastric cancer patients and its clinical significance.Methods The CK20 mRNA and CEA mRNA were detected by RT-PCR assay in peripheral blood samples from 52 patients with gastric cancer.Results The positive expression of CK20 mRNA and CEA mRNA were 42.3%(22/52) and 51.9%(27/52) respectively.Thirty-three(63.5%) blood samples had positive expression of one or both markers.The positive expression of CK20 mRNA correlated with distant metastasis.The positive expression of CEA mRNA,or CK20 mRNA,or both,closely related with pathological stage,lymph node metastases and distant metastasis(P<0.05).Conclusion It indicates that the combined detection of CK20 mRNA and CEA mRNA by RT-PCR assay may be useful for detecting micrometastasis and prognosis of the patients with gastric cancer.