| 小鼠原发性肝癌短期诱导模型的建立和评价 |
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| 引用本文： | 刘勇,赛岩,刘冬梅,刘杨,文川,范礼斌,崔春萍. 小鼠原发性肝癌短期诱导模型的建立和评价[J]. 军事医学科学院院刊, 2011, 35(3): 175-179,192 |
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| 作者姓名： | 刘勇 赛岩 刘冬梅 刘杨 文川 范礼斌 崔春萍 |
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| 作者单位： | 1. 安徽医科大学生命科学院生物学教研室,合肥,230032
2. 军事医学科学院放射与辐射医学研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金资助项目，国家重大新药创制重大专项资助项目 |
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| 摘 要： | 目的用一个月的时间建立小鼠原发性肝癌模型。方法用二乙基亚硝胺（DEN）通过腹腔注射的方式对C57BL/6小鼠进行肝脏物理损伤和毒性诱导,再经过30%部分肝切除（PH）刺激肝再生,同时辅以二乙酰基芴（2-AAF）抑制肝实质细胞分裂,使DEN的毒性诱导作用直接作用于肝脏卵圆细胞（OVC）,诱导突变。结果 模型组存活8只（8/12）,将存活的各组小鼠通过放射免疫分析（R IA）、免疫组化（IHC）和免疫印迹（WB）方法鉴定短期诱导小鼠原发性肝癌建模是否成功,结果发现原发性肝癌的绝大多数指标明显改变。结论鉴定结果表明采用化学损伤结合部分肝切除方法,经过一个月时间成功诱导并建立了C57BL/6小鼠的原发性肝癌模型。
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| 关 键 词： | 肝细胞癌 二乙基亚硝胺 肝切除术 肝再生 突变 二乙酰基芴 卵圆细胞 免疫组织化学 |
Establishment and evaluation of a model of short-term carcinogenesis for C57BL/6 mice |
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| LIU Yong,SAI Yan,LIU Dong-mei,LIU Yang,WEN Chuan,FAN Li-bin,CUI Chun-ping. Establishment and evaluation of a model of short-term carcinogenesis for C57BL/6 mice[J]. Bulletin of the Academy of Military Medical Sciences, 2011, 35(3): 175-179,192 |
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| Authors: | LIU Yong SAI Yan LIU Dong-mei LIU Yang WEN Chuan FAN Li-bin CUI Chun-ping |
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| Affiliation: | LIU Yong1,SAI Yan1,LIU Dong-mei2,LIU Yang2,WEN Chuan2,FAN Li-bin1,CUI Chun-ping2(1.Department of Biology,School of Life Sciences,Anhui Medical University,Hefei 230032,China,2.Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China) |
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| Abstract: | Objective To establish a primary hepatoma model of mice in one month.Methods Physical injury and toxicity were induced in the liver of C57BL/6 mice through DEN intraperitoneal injection,liver regeneration was provoked with 30% partial hepatectomy(PH),2AAF was used to inhibit cell division of hepatic parenchymal cells to make sure that DEN toxicity would directly affect the liver oval cells to induce the mutation of the liver oval cells.Results 8(8/12) mice survived in the experimental group.The survival of ... |
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| Keywords: | hepatocellular carcinoma diethylnitrosamine hepatectomy liver regeneration mutation 2-AAF oval cell immunohistochemistry |
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