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| 紫杉醇热敏脂质体制备及抗肿瘤作用评价 | |
| 引用本文： | 尤晓惠,张慧,潘见,付盟,梅兴国,胡富强. 紫杉醇热敏脂质体制备及抗肿瘤作用评价[J]. 军事医学科学院院刊, 2011, 35(3): 219-222 |
| 作者姓名： | 尤晓惠 张慧 潘见 付盟 梅兴国 胡富强 |
| 作者单位： | 1. 合肥工业大学农产品生物化工教育部工程研究中心,合肥,230009;军事医学科学院毒物药物研究所,北京,100850 2. 军事医学科学院毒物药物研究所,北京,100850 3. 合肥工业大学农产品生物化工教育部工程研究中心,合肥,230009 4. 浙江大学药学院,杭州,310058 |
| 基金项目： | 国家"973"计划资助项目，国家重大新药创制科技重大专项资助项目 |
| 摘 要： | 目的制备紫杉醇热敏脂质体（PTL）并考察其抗肿瘤作用。方法采用薄膜分散法制备PTL及紫杉醇普通脂质体（PL）;高效液相色谱（HPLC）法测定体内外紫杉醇的含量。建立小鼠Lewis肿瘤模型,随机分为4组,即对照组、紫杉醇注射液（PTX）组、PL组和PTL组,每组给药后均对肿瘤部位加热30 min,监测肿瘤的体积及小鼠生活状态,考察抑瘤效果。结果制备的PTL粒径分布在70~140 nm,包封率约为99%,相变温度约为42℃;体内热敏实验中,PTL组加热部位药物浓度明显高于PTX组和PL组加热部位;PTX组、PL组和PTL组的抑廇率分别为49.79%、57.40%和75.66%。结论与PTX和PL相比,PTL具有明显的热敏靶向性,与热疗相结合显著提高了紫杉醇的治疗效果。 |
| 关 键 词： | 紫杉醇 脂质体 热敏 抗肿瘤作用 |
Preparation of thermosensitive liposomes containing paclitaxel and their anticancer effect |
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| YOU Xiao-hui,ZHANG Hui,PAN-Jian,FU-Meng,MEI Xing-guo,HU Fu-qiang. Preparation of thermosensitive liposomes containing paclitaxel and their anticancer effect[J]. Bulletin of the Academy of Military Medical Sciences, 2011, 35(3): 219-222 | |
| Authors: | YOU Xiao-hui ZHANG Hui PAN-Jian FU-Meng MEI Xing-guo HU Fu-qiang |
| Affiliation: | YOU Xiao-hui1,2,ZHANG Hui2,PAN-Jian1,FU-Meng2,MEI Xing-guo2,HU Fu-qiang3(1.Engineering Research Center of Bio-process of Ministry of Education,Hefei University of Technology,Hefei 230009,China,2.Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,3.School of Pharmacy,Zhejiang University,Hangzhou 310058,China) |
| Abstract: | Objective To prepare the thermosensitive liposomes containing paclitaxel(PTL)and study their anticancer effect.Methods Both PTL and paclitaxel liposomes(PL) were prepared by the thin-film method.The HPLC method was established to determine paclitaxel concentration in vivo and in vitro.The model of mice Lewis sarcoma was established and divided into four groups randomly:control,PTX,PL and PTL groups.Each group was administrated with drug and local hyperthermia for 30 min.The volume and weight of tumor were d... |
| Keywords: | paclitaxel liposomes thermosensitivity anticancer effect |
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