氟哌利多加强针刺镇痛时大鼠脑内μ受体结合位点增加:放射自显影研究(英文)

目的:观察μ受体在氟哌利多加强针刺镇痛过程中的变化.方法:运用放射自显影技术,以μ受体的高选择性配基羟甲芬太尼在大鼠脑片上作放射受体结合分析.结果:针刺使~3H-羟甲芬太尼与mu受体的结合在大鼠脑内许多核团明显增加;当氟哌利多增强针刺镇痛时,这一结合进一步增加.增加程度较大的核团有尾核,伏核,视前区,中脑导水管周围灰质(P<0.01);中等程度的增加可见于丘脑,下丘脑外侧区,脊髓背角等(P<0.01或0.01);增加程度较小者有隔核,视前区,黑质(P<0.05).结论:μ受体介导了氟哌利多对针刺镇痛的加强作用.

Binding sites of mu receptor increased when acupuncture analgesia was enhanced by droperidol: an autoradiographic study

To study if u receptor participates in the process of potentiation of droperidol (Dro) on acupuncture analgesia (AA). METHODS : Autoradiographic technic was used. Ohmefentanyl, a highly selective ligand of u receptors, was used in radio-receptor binding assay in Sprague-Dawley rat brain sections. RESULTS: The binding sites of [B-3H, p-ben-zoyl-3H]ohmefentanyl were increased greatly in many nuclei of rat brain after AA, and were further increased when AA was enhanced by Dro. Higher increase was seen in caudate nucleus,accumbens, periaqueductal gray (PAG),interpeduncular nucleus, amygdala (P<0. 01 vs rats treated with electroacupunc-ture alone) ; moderate increase was noted in thalamus, lateral area of hypothalamus, spinal dorsal horn (P<0. 01 or 0. 05); slight increase appeared in septum, preoptic area, hippocampus, substantia nigra (P<0. 05). CONCLUSION: Mu opioid receptors mediated the Dro-induced enhancement of AA.