温胆汤对地卓西平马来酸盐诱发精神分裂症模型大鼠脑组织中Cx43 mRNA表达的影响

目的:探讨温胆汤对精神分裂症模型大鼠脑组织中神经胶质细胞连接蛋白(Cx43)mRNA的影响。方法:将健康的SD大鼠50只随机分为5组:正常组(A)、模型组(B)、温胆汤高、中、低剂量组(C,D,E组,剂量为生药40,20,10 g.kg-1)。A,B组生理盐水ig,C,D,E组温胆汤ig,1次/d,21 d后,按照0.6 mg.kg-1一次性ip地卓西平马来酸盐(MK-8O1)建立拟精神分裂症模型,然后行为学观察3 d,处死大鼠。应用逆转录聚合酶链式反应(RT-PCR)法,检测大鼠Cx43 mRNA的表达量。结果:模型组Cx43 mRNA的表达量低于正常组,有显著性意义(P<0.05);温胆汤3个剂量组Cx43 mRNA的表达量明显高于模型组,有显著性意义(P<0.05)。结论:温胆汤能够增强精神分裂症模型大鼠脑组织中Cx43 mRNA的表达。

Effect of Wendan Decoction on Connexin 43 mRNA Expression of in Rat Brain Tissue with Schizophrenia Induced by Dizocilpine Maleate

Objective:To study the effect of Wendan decoction on connexin 43(Cx43) mRNA expression in brain tissue of rats with schizophrenia. Method: Fifty SD rats were divided five groups by random, including normal group(A),model group(B),Wendan decoction high-dosage group(C),medium-dosage group(D) and low-dosage group(E),respectively. The A and B groups were given normal saline and the C,D, E groups were given Wendan decoction(40,20,10 g·kg^-1) every day, which lasted for 21 days. Dizocilpine maleate(MK-801) 0.6 mg·kg^-1 was ip given to establishthe rat schizophrenic model. Then rat behaviors were observed for 3 days all rats were sacrificed. Then RT-PCR method was used to detect Cx43 mRNA expression in the nervecells of the every group rats. Result: The Cx43 mRNA expression of group B was lower than that of group A (P<0.05).And the Cx43 mRNA expression of group C,D and E was rose compared with that of group B(P<0.05). Conclusion: Wendan decoction can reinforce the expression of Cx43 mRNA in the brain tissue of rats with schizophrenia.