薄荷饮片挥发油气相色谱指纹图谱研究

目的建立薄荷饮片挥发油的气相色谱(GC)指纹图谱。方法采用HP-INNOWax毛细管柱(30 m×0.32 mm,0.25μm),进样口温度250℃,检测器温度为280℃,炉温采用程序升温,初始温度100℃,保持15 min后,以8℃/min升至140℃后保持10 min,再以5℃/min升至200℃,载气流速为1.3 mL/min,分流比为2:1。结果 10批薄荷饮片挥发油标示出13个共有峰,5号峰(薄荷脑)的峰面积百分含量为(65.78±13.90)%。结论该方法准确可靠、重复性好,为薄荷饮片质量控制提供了一定的参考。

Study on GC Fingerprint of Volatile Oil of Decoction Pieces of Mentha Haplocalyx Herba

Objective To establish the method of fingerprint analysis on volatile oil of the decoction pieces of Mentha Haplocalyx Herba by GC.Methods The HP-INNOWax capillary column（30 mm×0.32 mm,0.25 μm） was used with the sampling temperature at 250 ℃ and the FID detector temperature at 280 ℃.The temperature programming was adopted with the initial column temperature at 100 ℃,which was kept for 15 min,then increased by 8 ℃/min^-1 to 140 ℃,after keeping for 10 min,reincreased by 5 ℃/min^-1 to 200 ℃.The flow rate of the carrier gas was 1.3 mL/min^-1 with the split ratio of 2 ∶1.Results 13 common peaks were separated on GC fingerprint spectrum in 10 batches of the decoction pieces of Mentha Haplocalyx Herba.The percentage composition of peak area in peak 5（menthol） was（65.78±13.90）%.Conclusion The method is reliable and accurate with good reproducibility,which provides certain reference for controlling the quality of Mentha Hapiocalycx Herba.