| 死亡受体5氨基末端的结构与功能 |
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| 作者姓名： | Feng Z Cai-feng C Shi-lian L De-xian Z Yan-xin L |
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| 作者单位： | 中国医学科学院北京协和医学院基础医学研究所医学分子生物学国家重点实验室,北京,100005 |
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| 基金项目： | 国家重点基础研究发展计划项目(973计划),国家自然科学基金 |
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| 摘 要： | 目的 研究死亡受体5(DR5)氨基末端的结构与功能.方法 利用基因工程技术构建一系列DR5胞外区(DR5-ECD)缺失突变体并获得相应的重组蛋白.利用蛋白免疫印迹及ELISA技术研究重组蛋白与小鼠抗人DR5单克隆抗体AD5-10的结合情况.结果 含有完整氨基末端结构的DR5-ECD缺失突变体能够与AD5-10结合,而缺失氨基末端的重组蛋白则不能与AD5-10相互作用.结论 DR5氨基末端含有AD5-10激动性抗体的靶点,该靶点可能在发展肿瘤免疫治疗新策略中发挥重要作用.
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| 关 键 词： | 死亡受体 激动性抗体 肿瘤治疗 |
Structural and functional analysis of the N-terminal region of death receptor 5 |
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| Feng Z,Cai-feng C,Shi-lian L,De-xian Z,Yan-xin L.Structural and functional analysis of the N-terminal region of death receptor 5[J].Acta Academiae Medicinae Sinicae,2011,33(1):33-38. |
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| Authors: | Feng Zhang Cai-feng Chen Shi-lian Liu De-xian Zheng Yan-xin Liu |
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| Institution: | ZHANG Peng,CHEN Cai-feng,LIU Shi-lian,ZHENG De-xian,LIU Yan-xin National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China |
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| Abstract: | Objective To investigate the structure and function of the N-terminal region(NTR) of death receptor 5(DR5).Methods A series of deletions of the DR5 extracellular domain(DR5-ECD) proteins were expressed in E.coli.and purified by affinity chromatography.The binding ability of these deletant proteins to AD5-10,a mouse anti-human DR5 monoclonal antibody,was evaluated by immunoblotting and ELISA.Results Recombinant DR5-ECD proteins containing the NTR were recognized and bound by AD5-10,while the other deletant p... |
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| Keywords: | death receptor 5 agonistic antibody tumor therapy |
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