| 反相高效液相色谱法测定洁白胶囊中没食子酸含量 |
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| 引用本文： | 王景,徐秀珍,夏文孝,李小萍.反相高效液相色谱法测定洁白胶囊中没食子酸含量[J].中国药业,2011,20(2):41-42. |
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| 作者姓名： | 王景 徐秀珍 夏文孝 李小萍 |
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| 作者单位： | 1. 宁夏多维药业有限公司,宁夏,银川,750101
2. 宁夏药品检验所,宁夏,银川,750100 |
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| 摘 要： | 目的建立测定洁白胶囊中没食子酸含量的反相高效液相色谱法。方法以5C18-MS-Ⅱ柱(250 mm×4.6 mm,5μm)为固定相,甲醇-水-磷酸(6∶94∶0.4)为流动相,检测波长272 nm。结果没食子酸进样质量浓度在3.32～39.84μg/mL范围内与峰面积积分值线性关系良好,r=0.999 9(n=5);平均回收率为99.80%,RSD=2.50%(n=6)。结论该方法测定简便、快速、灵敏、重现性好,可用于测定洁白胶囊中没食子酸的含量。
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| 关 键 词： | 反相高效液相色谱法 洁白胶囊 没食子酸 含量测定 |
Determination of Gallic Acid in Jiebai Capsules by RP-HPLC |
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| Wang Jing,Xu Xiuzhen,Xia Wenxiao,Li Xiaoping.Determination of Gallic Acid in Jiebai Capsules by RP-HPLC[J].China Pharmaceuticals,2011,20(2):41-42. |
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| Authors: | Wang Jing Xu Xiuzhen Xia Wenxiao Li Xiaoping |
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| Institution: | Wang Jing1,Xu Xiuzhen2,Xia Wenxiao1,Li Xiaoping1(1.Ningxia Duowei Pharmaceutical Co.,Ltd.,Yinchuan,Ningxia,China 750101,2.Ningxia Institute for Drug Control,China 750100) |
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| Abstract: | Objective To establish a RP-HPLC method for the determination of gallic acid in Jiebai Capsules.Methods To determine the gallic acid content by HPLC with the 5C18-MS-Ⅱcolumn（250 mm×4.6 mm,5 μm） as the stationary phase.The mobile phase was methanol-water-phosphoric acid（6 ∶94 ∶0.4）.The detection wavelength was 272 nm.Results The linear range of gallic acid was 3.32-39.84 μg/mL,r= 0.999 9（n=5）.The average recovery rate was 99.81%,RSD 2.50%（n=6）.Conclusion The method is fast,simple,sensitive and repeatable,which can be used to determine the gallic acid content in Jiebai Capsules. |
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| Keywords: | RP-HPLC Jiebai Capsules gallic acid content determination |
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