| 高效液相色谱法测定生发素中米诺地尔含量 |
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| 引用本文： | 邓莉,林彩,胡斌,赵映兰,刘波.高效液相色谱法测定生发素中米诺地尔含量[J].中国药业,2011,20(3):18-19. |
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| 作者姓名： | 邓莉 林彩 胡斌 赵映兰 刘波 |
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| 作者单位： | 第三军医大学西南医院药剂科,重庆,400038 |
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| 摘 要： | 目的建立测定生发素中米诺地尔含量的高效液相色谱法。方法采用C18色谱柱(200mm×4.6mm,5μm),流动相为0.03mol/L的K2HPO4溶液(pH=3.3)-甲醇(55∶45),检测波长287 nm,流速0.9 mL/min,柱温30℃,进样量10μL。结果米诺地尔质量浓度在2.16～15.12μg/mL范围内与峰面积线性关系良好(r=0.999 96),平均回收率为99.36%,RSD为1.21%(n=6)。结论高效液相色谱法简便、准确、灵敏,适用于生发素中米诺地尔的含量测定。
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| 关 键 词： | 生发素 米诺地尔 高效液相色谱法 含量测定 |
Determination of Minoxidil in Shengfasu by HPLC |
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| Deng Li,Lin Cai,Hu Bin,Zhao Yinglan,Liu Bo.Determination of Minoxidil in Shengfasu by HPLC[J].China Pharmaceuticals,2011,20(3):18-19. |
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| Authors: | Deng Li Lin Cai Hu Bin Zhao Yinglan Liu Bo |
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| Institution: | Deng Li,Lin Cai,Hu Bin,Zhao Yinglan,Liu Bo(Department of Pharmacy,Southwest Hospital,Third Military Medical University,Chongqing,China 400038) |
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| Abstract: | Objective To establish the determination method of the minoxidil content in Shengfasu.Methods The assay was conducted on the C18 column with 0.03 mol/L K2HPO4（pH=3.3）-methanol（55 ∶45） as the mobile phase.The detection wavelength was 287 nm and the flow rate was at 0.9 mL/min.The column temperature was 30 ℃,the sample size was 10 μL.Results The linear concentration range of minoxidil was 2.16-15.12 μg/mL（r=0.999 96） with the average recovery rate of 99.36%,RSD=1.21%（n=6）.Conclusion The method is simple and accurate,sensitive and reproducible,which can be used for the determination of minoxidil in Shengfasu. |
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| Keywords: | Shengfasu minoxidil HPLC content determination |
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