| 高效液相色谱法测定杏杷止咳颗粒中黄芩苷含量 |
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| 引用本文： | 陈珂,宋岚,邝明,陈菊.高效液相色谱法测定杏杷止咳颗粒中黄芩苷含量[J].中国药业,2011,20(1):26-27. |
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| 作者姓名： | 陈珂 宋岚 邝明 陈菊 |
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| 作者单位： | 1. 四川省泸州市食品药品检验所,四川,泸州,646000
2. 泸州医学院附属中医院,四川,泸州,646000 |
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| 摘 要： | 目的建立测定杏杷止咳颗粒中黄芩苷含量的高效液相色谱法。方法采用Agilent C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(50∶50∶0.2),流速为1.0 mL/min,检测波长为280 nm,柱温为室温。结果黄芩苷进样量在4～44μg(r=0.999 9)范围内与峰面积线性关系良好,平均加样回收率为99.69%,RSD=0.61%(n=6)。结论高效液相色谱法灵敏度高,操作简便,准确可靠,可用于杏杷止咳颗粒中黄芩苷的含量测定。
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| 关 键 词： | 杏杷止咳颗粒 黄芩苷 高效液相色谱法 含量测定 |
Determination of Baicalin in Xingpa Zhike Granules by HPLC |
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| Chen Ke,Song Lan,Kuang Ming,Chen Ju.Determination of Baicalin in Xingpa Zhike Granules by HPLC[J].China Pharmaceuticals,2011,20(1):26-27. |
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| Authors: | Chen Ke Song Lan Kuang Ming Chen Ju |
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| Institution: | Chen Ke1,Song Lan2,Kuang Ming1,Chen Ju1(1.Luzhou Institute for Food and Drug Control,Luzhou,Sichuan,China 646000,2.Affiliated Hospital of Chinese Medicine,Luzhou Medical College,Sichua n,China 646000) |
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| Abstract: | Objective To develop an HPLC method for the determination of baicalin in Xingpa Zhike Granules.Methods The Agilent C18 column（250 mm×4.6 mm,5 μm） was used with the mobile phase of methanol-water-phosphoric acid（50 ∶50 ∶0.2） at the flow rate of 1.0 mL/min.The UV detection was 280 nm and the temperature was 30 ℃.Results The linear relation of baicalin was well in the injection volume of 4-44 μg（r=0.999 9）.The average recovery rate was 99.69%,RSD=0.61%（n=6）.Conclusion The method is sensitive,convenient and accurate,which can be used to determine the content of baicalin in Xingpa Zhike Granules. |
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| Keywords: | Xingpa Zhike Granules baicalin HPLC content determination |
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