雪旺氏细胞基底膜成份-Laminin在神经再生中的作用探讨Laminin抗血清的活体应用及观察

实验用74只近交克隆系Wistar大鼠.切取供体神经,经冷冻和加热处理后,半数神经用正常兔血清处理,另半数用抗Laminin抗血清处理,然后分别植入对照组和实验组鼠体内.术后不同时间的电镜观察和统计数据表明:对照组再生轴突几乎都走在基底膜管内侧(92％),而实验组走在基底膜管内侧的轴突数(48％)与走在基底膜管外侧的轴突数(52％)几乎相等;轴突总数15天时实验组约为照对组的50％,30天时38％.实验组内轴突的长出时间较对照组晚约3天左右;实验组内巨噬细胞的数量和滞留时间均多于和长于对照组.这些结果表明Laminin在神经再生过程中不仅促进轴突生长成熟,引导神经方向性生长,亦对维持正常的再生微环境起了重要作用.

The effects of Schwann cell basal lamina on nerve regeneration. Experimental studies with anti-laminin antiserum

74 close cloned Wistar rats were divided into three groups ( donor, control and experimental), 1cm nerve segments of sciatic nerve from the donor rats were frozen and thawed to kill Schwann cells. Then, the grafts for the control recipient rats were treated with normal rabbit serum and the grafts for the experimental group of rats were doubly treated with normal serum and rabbit anti-laminin antiserum.In the control group, 92% regenerating axoas grew inside the basal lamina scaffolds, while in the experimental ( anti-laminin antiserum treated) group, the axons presented outside (52%) and inside (48%) the scaffolds simultaneously. The maximum number of axons in the experimental group was 50% of that in the control group at 15 days and 38% of that at 30 days after operation, and the growth was . postponed for 3 days. The results indicate that basal laminin scaffolds may play an important role on neurotrophic function as well as on maintaining the normal microenvironment for axon regeneration.