舒脉胶囊对大鼠缺血心肌促血管新生的影响

目的观察舒脉胶囊对缺血心肌血管新生和相关因子表达的影响。方法复制大鼠心肌缺血模型，分别给予舒脉胶囊（舒脉组）、贝复济＋肝素（阳性药组）、生理盐水（模型组），另设假手术组，给予生理盐水；给药不同时间（1、2、4周）后，用免疫组织化学法测定缺血心肌血管性血友病因子（vwF）、血管内皮细胞生长因子（VEGF）的蛋白表达；图像分析系统计算缺血心肌新生血管数；实时定量PCR检测VEGF的基因表达水平。结果与假手术组、模型组比较，舒脉组和阳性药组3个时间段新生微血管数显著增加（P〈0．01），两用药组比较，差异无统计学意义；与假手术组、模型组比较，舒脉组和阳性药组3个时间段VEGF基因及蛋白表达增加（P〈0．01），两用药组比较，差异无统计学意义。结论舒脉胶囊能促进实验性大鼠缺血心肌微血管生成，对VEGF基因及蛋白表达的上调可能是其促血管新生的作用机制之一。

Experimental Study on Effect of Shumai Capsule in Promoting Angiogenesis in Rats with Myocardial Ischemia

Objective To study the effect of Shumai Capsule (SMC) on angiogenesis and expression of relevant growth factor in rats with myocardial ischemia (MI). Methods Model rats of MI were duplicated and treated with SMC (SMC group), bFGF calparine (positive control group) and normal saline (model group) respectively. Besides, a sham-operative group was set up and treated with normal saline. The rats were sacrificed in batches at the time after being medicated for 1, 2 and 4 weeks, for determining von Willebrand factor (vWF) and vascular endothelial growth factor (VEGF) protein expression in ischemic myocardium by immunohistochemical staining, myocardial micro-vessel density (MVD) using digital analysis system, and the gene expression of VEGF by quantitative real-time PCR. Results Compared with the sham-operative group and the model group, levels of MVD, protein and gene expression of VEGF in the SMC group were higher respectively at three time segments (all P<0.01), but showed insignificant difference to those in the positive control group. Conclusion SMC could promote angiogenesis in ischemic myocardium of rats, the up-regulation on VEGF mRNA and protein expression might be one of the potential mechanisms of SMC in promoting angiogenesis.