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| 骨髓间充质干细胞向软骨细胞分化过程中miR130α的表达 | |
| 引用本文： | 苏金梅,金晔,曲强,张奉春,唐福林.骨髓间充质干细胞向软骨细胞分化过程中miR130α的表达[J].基础医学与临床,2010,30(5):520-523. |
| 作者姓名： | 苏金梅 金晔 曲强 张奉春 唐福林 |
| 作者单位： | 1. 中国医学科学院,北京协和医学院,北京协和医院风湿免疫科,北京,100032 2. 中国医学科学院,北京协和医学院,北京协和医院中心实验室,北京,100032 3. 中国医学科学院,北京协和医学院,北京协和医院基本外科,北京,100032 |
| 摘 要： | 目的诱导骨髓间充质干细胞向软骨细胞分化,初步探索miR130a在骨髓间充质干细胞向软骨细胞分化过程中的调控作用。方法体外TGF-β1诱导骨髓间充质干细胞向软骨细胞分化,免疫荧光法和免疫组化染色鉴定Ⅱ型胶原,阿辛兰染色鉴定氨基葡聚糖。用Real-time PCR法检测细胞miR130a的表达。结果骨髓间充质干细胞在TGF-β1诱导下可分化为软骨细胞。培养7 d后,诱导培养组细胞miR130a表达的水平显著低于培养前的水平(P<0.05)。结论骨髓间充质干细胞体外诱导可以分化为软骨细胞,在向软骨细胞分化的早期,miR130a表达降低伴随着软骨细胞的分化,提示与软骨形成的过程有关。 |
| 关 键 词： | 骨髓间充质干细胞 软骨细胞 分化 microRNA |
| 收稿时间： | 2009-12-23 |
| 修稿时间： | 2010-1-6 |
The expression of micro RNA130a on rat bone mesenchymal stem cells during chondrogenic differentiation |
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| SU Jin-mei,JIN Ye,QU Qiang,ZHANG Feng-chun,TANG Fu-lin.The expression of micro RNA130a on rat bone mesenchymal stem cells during chondrogenic differentiation[J].Basic Medical Sciences and Clinics,2010,30(5):520-523. | |
| Authors: | SU Jin-mei JIN Ye QU Qiang ZHANG Feng-chun TANG Fu-lin |
| Institution: | 1.Dept.of Rheumatology and Immunology;2.Research Center Laboratory;3.Dept.of General Surgery;PUMC Hospital;CAMS & PUMC;Beijing 100032;China |
| Abstract: | Objective To evaluate the role of microRNA130α on rat bone mesenchymal stromal cells(BMSCs)during chondrogenic differentiation.Methods BMSCs were induced to differentiate into chondroeytes by transforming growth factor-β1(TGF-β1)in vitro,immunofluorescence and immunohistochemistry were performed to evaluate MSCs differentiation.RT-PCR was performed to analyze microRNA130α expression at different time points.Results microRNA130α was down-modulated during chondrogenesis after BMSCs been cultured with TGF-β1 for 7 days (P ＜0.05).Conclusion During the early stage of BMSC chondrogenic differentiation,mciroRNA130a expression was specifically repressed,suggesting its role in differentiation of rat bone mesenchymal stromal cells. |
| Keywords: | bone mesenchymal stem cell chondrocyte differentiation microRNA |
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