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Sequence Conservation of the Region Targeted by the Abbott RealTime HBV Viral Load Assay in Clinical Specimens |
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| Authors: | Gavin A. Cloherty James Rhoads Thomas P. Young Neil T. Parkin Vera Holzmayer Lilly Yuen Carolyn Mullen |
| Affiliation: | aAbbott Molecular, Des Plaines, Illinois, USA;bData First Consulting, Belmont, California, USA;cUniversity of California, San Francisco, San Francisco, California, USA;dAbbott Diagnostics, Abbott Park, Illinois, USA;eEvivar Medical Pty. Ltd., Melbourne, Victoria, Australia |
| Abstract: | The Abbott RealTime HBV assay targets the N-terminal region of the S gene. Here we analyzed the sequence variability of the assay target region from >2,100 clinical specimens. Thermodynamic modeling of the percentage of bound primer/probe at the assay annealing temperature was performed to assess the potential effect of sequence variability. |
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