Detection of Pneumocystis jirovecii by PCR in patients with lung cancer: A preliminary study |
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| Institution: | 1. Microbiology Laboratory, Ataturk Training and Research Hospital, Izmir Katip Celebi University, Turkey;2. Institute of Health Sciences, Department of Basic Oncology, Ege University, Izmir, Turkey;3. Chest Disease Department, Dr. Suat Seren Chest Disease and Surgery Training and Research Hospital, Izmir, Turkey;4. Translational Pulmonary Research Group (EGESAM), Ege University, Izmir, Turkey;5. Faculty of Medicine Department of Parasitology, Ege University, Izmir, Turkey;6. Faculty of Medicine Department of Chest Disease, Ege University, Izmir, Turkey;1. Division of Respiratory Medicine, Department of Pediatrics, The Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto M5G 1X8, Canada;2. Child Evaluative Health Sciences, Research Institute, The Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto M5G 1X8, Canada;3. Division of Infectious Diseases, Department of Pediatrics, The Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto M5G 1X8, Canada;4. Division of Microbiology, Department of Pediatric Laboratory Medicine, The Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto M5G 1X8, Canada;5. Division of Respirology, Department of Medicine, St. Michael''s Hospital, University of Toronto, 30 Bond Street, Toronto M5B 1W8, Canada;6. Keenan Research Centre of Li Ka Shing Knowledge Institute, Department of Medicine, St. Michael''s Hospital, University of Toronto, 30 Bond Street, Toronto M5B 1W8, Canada;1. Center of Clinical Laboratory Science, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Nanjing 210009, China;2. The Fourth Clinical School of Nanjing Medical University, Nanjing 210029, China;3. Department of Oncology, Xuzhou Medical College, Xuzhou 221004, China;4. Department of General Surgery, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Nanjing 210009, China;1. Oncology Center, Affiliated Hospital of Guangdong Medical University, No. 57 Peoples Avenue South, Zhanjiang, Guangdong 524001, People''s Republic of China;2. Department of Radiation Oncology, Central Hospital of Guangdong Nongken, No. 2 Peoples Avenue, Zhanjiang, Guangdong 524022, People''s Republic of China;3. Department of Urology, Central Hospital of Guangdong Nongken, No. 2 Peoples Avenue, Zhanjiang, Guangdong 524022, People''s Republic of China |
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| Abstract: | IntroductionInfection complications in lung cancer (LC), one of the most common cancers in the world, are still among the most important causes of death. Of them, P. jirovecii, which is as an opportunistic infection, causes a life-threatening type of pneumonia in cancer patients. This preliminary study aimed to determine the incidence and clinical status of P. jirovecii by PCR in lung cancer patients compared to the conventional method.Material and methodsSixty-nine lung cancer patients and fSorty healthy individuals were included in the study. After sociodemographical and clinical features were recorded, sputum samples were collected from attenders. Firstly, microscopic examination was made with Gomori's methenamine silver stain and then PCR was performed.ResultsP. jirovecii was detected in three of 69 lung cancer patients by PCR (4.3%), but not by microscopy. However, healthy individuals were negative for P. jirovecii by both methods. Based on clinical and radiological findings, P. jirovecii was evaluated as probable infection in one patient and colonization in the other two patients. Although PCR is more sensitive than conventional staining methods, it cannot distinguish probable and proven infections from pulmonary colonization.DiscussionIt is important to evaluate the decision of infection together with laboratory, clinical and radiological findings. Moreover, PCR may enable to know the colonization and to take precautions such as prophylaxis, due to the risk of colonization turning into an infection in immunocompromised patient groups. Further studies involving larger populations and evaluating the colonization-infection relationship in patients with solid tumors are needed. |
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| Keywords: | Polymerase chain reaction |
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