| CTLA4—lg融合蛋白基因的表达及其生物学功能的初步研究 |
| |
| 引用本文： | 郑宇,沈倍奋.CTLA4—lg融合蛋白基因的表达及其生物学功能的初步研究[J].细胞与分子免疫学杂志,2000,16(1):14-17. |
| |
| 作者姓名： | 郑宇 沈倍奋 |
| |
| 基金项目： | 国家八六三计划生物技术资助!No.102 09 01 03 |
| |
| 摘 要： | 获得ＣＴＬＡ４全长基因并在真核表达系统中表达。方法通过ＲＴ－ＰＣＲ方法，从ＭＴ２细胞系克隆ＣＴ－ＬＡ４全长基因；经直物重叠和半巢式ＰＣＲ改造，获得含抑瘤素Ｍ信号肽的ＣＴＬＡ４胞外段ＤＮＡ，然后克隆至真核表达载体ｐＩｇ，转染ＣＯＳ和ＣＨＯ－Ｋ细胞表达。结论真核表达体系表达了有生物学活性的ＣＴＬＡ４－Ｉｇ。
|
| 关 键 词： | CTLA4 CTLA4－Ig 克隆 表达 |
| 修稿时间： | 1999－02－12 |
Expression
and biological activities of CTLA4-Ig fusion protein |
| |
| ZHENG Yu,SHEN Bei feng,LI Yan,SHU Cui ling,HU Mei ru,PEI Wu hong,WANG Jian an Institute of Basic Medical Sciences,Acadamy of Military Medical Sciences,Beijing ,China.Expression
and biological activities of CTLA4-Ig fusion protein[J].Journal of Cellular and Molecular Immunology,2000,16(1):14-17. |
| |
| Authors: | ZHENG Yu SHEN Bei feng LI Yan SHU Cui ling HU Mei ru PEI Wu hong WANG Jian an Institute of Basic Medical Sciences Acadamy of Military Medical Sciences Beijing China |
| |
| Institution: | ZHENG Yu,SHEN Bei feng,LI Yan,SHU Cui ling,HU Mei ru,PEI Wu hong,WANG Jian an Institute of Basic Medical Sciences,Acadamy of Military Medical Sciences,Beijing 100850,China |
| |
| Abstract: | Aim To obtain and express the CTLA4 gene in eukaryocytes. Methods The full gene of the CTLA4 was amplified from MT2 cell lines by RT PCR, reformed with an overlapping primers and nested primers, then cloned into the expression vector pIg, consequently, the CTLA4 Ig was expressed in the COS and CHO K after transfection with pIg CTLA4. Results Sequencing data indicated that the CTLA4 gene was exactly identical to the sequence in the GenBank. The CTLA4 Ig was expressed in the COS and CHO K and it blocked the lymphocyte transformation. Conclusion The CTLA4Ig with biological activity was expressed in the eukaryotic system. |
| |
| Keywords: | CTLA4 CTLA4 Ig clone expression |
| 本文献已被 维普 等数据库收录！ |
|
