乳兔雪旺细胞对成兔视神经挫伤修复的作用

目的 研究乳兔雪旺细胞(Schwann cell,SC)对成兔视神经挫伤修复的作用。 方法 建立成兔视神经挫伤模型，伤后24 h分别向伤眼玻璃体腔内注入SC悬液(A组)、生理盐水(B组)各0.1 ml。伤后不同时间点进行视网膜节细胞(retinal ganglion cell,RGC)、轴突染色记数及闪光视觉诱发电位(flash visual evoked potentials,FVEP)检测。 结果 伤后4周A、B组RGC平均记数分别为(19.89±3.79) /mm和(12.67±4.12) /mm，轴突密度分别为(94.569±793) /mm ²和(36.085±285) /mm²，A组明显高于B组(P<0.01)。伤后3 d A组伤眼与健眼FVEP幅值比由48%上升至88%，8周时仍为78%，各时间点均明显高于B组(P＜0.01)。 结论 乳兔SC能够提高成兔视神经挫伤后RGC存活率,减轻轴突变性,显著促进视神经功能恢复,对视神经挫伤修复具有明显的促进作用。(中华眼底病杂志,2000,16:91-93) 

Effects of neonatol rabbit Schwann cells on promoting repair of optic nerve contusion in adult rabbits

Objective To study the effects of neonatol rabbit Schwann cells(SC) on repair of optic contusion in adult rabbits. Methods 24 h after the adult rabbit optic nerves was contused,0.1 ml of SC suspension　(group A)　and saline water　(group B)　were injected into the vitreous of injured eyes respectively.All the animals were studied by retinal ganglion cell　(RGC) and axon counting,flash visual evoked potential　(FVEP)　tests at various intervals after injury. Results At the 4th week after injury,the number of RGC was　(19.89±3.79)/mm in group A and (12.67±4.12)/mm in group B,and the density of axons was　(94.569±793)/mm² in group A and (36.085±285)/mm² in group B.There was dramatical difference between group A and B　(P<0.01).The amplitude of FVEP wave of group A increased from 48% to 88% on the 3rd day after injury,and still dept 78% at the 8th week and group A was significantly higher than group B at various intervals　(P<0.01). Conclusion SC are effective in promoting the repair of optic nerve contusion by increasing the survival rate of RGC,rescuing axons from degeneration,and dramatically promoting the function of the optic nerve. (Chin J Ocul Fundus Dis,2000,16:91-93)