Cloning and characterization of a chitinase (CHIT42) cDNA from the mycoparasitic fungus Trichoderma harzianum |
| |
Authors: | Irene García José M Lora Jesús de la Cruz Tahía Benítez Antonio Llobell José A Pintor-Toro |
| |
Institution: | (1) Instituto de Recursos Naturales y Agrobiología, CSIC, Apdo., E-1052, Sevilla, Spain;(2) Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC, Apdo. E-1113, Sevilla, Spain;(3) Universidad de Sevilla, Apdo. E-1113, Sevilla, Spain;(4) Departamento de Genética, Universidad de Sevilla, Apdo., E-1095, Sevilla, Spain |
| |
Abstract: | A cDNA of Trichoderma harzianum (chit42), coding for an endochitinase of 42 kDa, has been cloned using synthetic oligonucleotides corresponding to aminoacid sequences of the purified chitinase. The cDNA codes for a protein of 423 amino acids. Analysis of the N-terminal amino-acid sequence of the chitinase, and comparison with that deduced from the nucleotide sequence, revealed post-translational processing of a putative signal peptide of 22 amino acids and a second peptide of 12 amino acids. The chit42 sequence presents overall similarities with filamentous fungal and bacterial chitinases and to a lesser extent with yeast and plant chitinases. The deduced aminoacid sequence has putative catalytic, phosphorylation and glycosylation domains. Expression of chit42 mRNA is strongly induced by chitin and chitin-containing cell walls and is subjected to catabolite repression. Southern analysis shows that it is present as a single-copy gene in T. harzianum. chit42 is also detected in several tested mycoparasitic and non-mycoparasitic fungal strains. |
| |
Keywords: | Trichoderma Chitinase Mycoparasitism Biological control |
本文献已被 SpringerLink 等数据库收录! |
|