ZnT3与Aβ在APP/PS1转基因小鼠脑血管及脉络丛的一致性分布

目的研究锌转运蛋白3(Zinc Transporter 3,ZnT3)与β-淀粉样蛋白(β-amyloid,Aβ)在APP/PS1转基因小鼠大脑血管壁及脉络丛上皮的定位分布,探讨ZnT3影响脑锌平衡从而参与AD发病的可能机制。方法应用免疫荧光技术和共聚焦激光扫描显微镜观察ZnT3和Aβ在APP/PS1转基因小鼠大脑血管壁及脉络丛上皮的共存情况。结果APP/PS1转基因小鼠侧脑室及第三、四脑室的脉络丛上皮细胞均呈ZnT3和Aβ染色阳性,二者共同表达于上皮细胞的胞质内,而细胞核未见任何着色。在APP/PS1转基因小鼠大脑皮层中,几乎所有Aβ阳性的血管壁上均有ZnT3的表达,二者的分布同样具有一致性。结论ZnT3与Aβ在APP/PS1转基因小鼠大脑血管及脉络丛上皮的一致性分布,提示ZnT3可能参与Aβ在大脑血管及脉络丛上皮的沉积。

Coincident Distribution of ZnT3 and Aβ in the Cerebral Vessels and Choroid Plexus of APP/PS1 Transgenic Mouse

Object To investigate the localizations of zinc transporter 3(ZnT3) andβ-amyloid protein(Aβ)in the cerebral vessels and choroid plexus of APP/PS1 transgenic mouse and to explore the possible role of ZnT3 in AD pathogenesis. Methods Immunofluorescence and confocal laser scanning microscopy were used to analyze the coincident distribution of ZnT3 and Aβ in the cerebral vessels and choroid plexus of APP/PS1 transgenic mouse. Results In the APP/PS1 transgenic mouse, the epithelial cells of choroid plexus of the lateral, third, and fourth ventricles presented both Aβ and ZnT3 positive immunoreactivity in the cytoplasm, but absent in the nucleus. ZnT3 immunoreactivity was also observed in almost all the Aβ-positive wall of blood vessels. Conclusion The coincident distributions of ZnT3 and Aβ immunoreactivity in the cerebral vessels and choroid plexus suggest that ZnT3 may be involved in the deposition of Aβ in the cerebral vessels and choroid plexus.