驻极体外电场对季铵化壳聚糖生长因子纳米粒制备的影响

目的 研究驻极体外电场对季铵化壳聚糖生长因子纳米粒制备及表征的影响,探究季铵化壳聚糖生长因子纳米粒的经皮载药转运机制.方法 以表皮生长因子为模型药物、季铵化壳聚糖为材料、三聚磷酸钠为交联剂,在不同表面电位驻极体产生的外静电场作用下,采用离子交联法制备不同电场条件下的季铵化壳聚糖生长因子纳米粒.透射电镜观察纳米粒的表面形态,粒径-zeta电位仪测定纳米粒的粒径和zeta电位.结果 (1)用离子交联法制备的季铵化壳聚糖生长因子纳米粒呈球形,分布均匀、分散性较好,且具有良好的稳定性.(2)季铵化壳聚糖生长因子纳米粒的粒径随季铵化壳聚糖浓度的增加而增大,季铵化壳聚糖的浓度可能是调控季铵化壳聚糖生长因子纳米粒粒径的主要因素.(3)外静电场能引起壳聚糖生长因子纳米粒极化.季铵化壳聚糖生长因子纳米粒的粒径随静电场场强的增大而减小,zeta电位的变化也与外加电场的极性密切相关.(4)经静电场处理后的季铵化壳聚糖生长因子纳米粒的粒径随存放时间的推移而减小.结论 季铵化壳聚糖生长因子纳米粒的形态、粒径和zeta电位与外加电场的场强大小和极性密切相关,要制备粒径适当、稳定性好的纳米粒,必须合理选择电场参数.

Effect of electret electrostatic-field on preparing nanopaticles carrying quaternary ammonium chitosan and epidermal growth factor

_{To the study of the influence of preparation and characterization of EGF nanoparticles and explore the percutaneous transfer mechanism of drug-loading nanoparticles. In this paper,Based on the basic EGF as model drug, quaternary ammonium chitosan as material,sodium tripolyphosphate as crosslinking agent, using the electret electrostatic field toPrepare EGF-loading chitosan nanoparticle solution.By means of transmission electron microscope to observe the surface morphology under different conditions. The results showed that: (1) The EGF-loading chitosan nanoparticles has uniform distribution, good dispersibility and stability. (2) The EGF can be polarized by electrostatic field.(3) The particle size of EGF-loading chitosan nanoparticles decreases with the intensity of the electrostatic field;the change of the zeta potential is closely related to the polarity of the applied electric field .(4)After the electric field treatment of chitosan nanoparticles,its particle size decreases with storage time.Result: The size and surface properties of chitosan nanoparticles is closely related to the applied electric field strength and polarity, to prepare the good quality chitosan nanoparticles,must choose a reasonable electric field parameters.}