乙型肝炎病毒基因分型的标签序列研究

目的 快速、准确地鉴定HBV基因型,寻找基因型间特异性的序列标签.方法 通过生物信息学手段,比对分析美国国立生物技术信息中心数据库中930条各型HBV基因组序列的大S区域,选择型间具有高度特异性的序列片段作为分型标签序列,以此设计引物,通过PCR实验对重庆地区的80例临床HBV阳性血清样本进行分型观察. 结果 HBV基因组大S区的149～169 nt和461-483 nt两段区域含有多个保守的型间差异位点;在8种基因型标签对构成的28种型组合中,27种组合的碱基总差异数均≥6;PCR实验和电泳结果证实,来自该两区段的序列标签引物能够获得特异性的产物,有效鉴定出HBV基因型.结论 HBV基因大S区的149～169 nt和461～483 nt两段序列可作为HBV基因型分型标签序列,用作引物探针设计或测序标签.

Identification of HBV genotype-specific tag sequences

Objective To indentify the HBV genotype-specific tag sequence. Methods The large S region sequences from 930 HBV genomes were aligned to identify the genotype-specific tag sequences. PCR was used to check the genotyping effect of these tags. Results Two tag sequences, sequence between 149-169 and sequence between 461-483, were identified in the large S region. Using primers specific to these tag sequences, the genotype of HBV can be specifically identified. Conclusion These tag sequences can be used for HBV genotyping.