吸毒合并HIV感染与HLA-DRB1*1301/1302等位基因的相关性

目的 探讨吸毒并HIV感染与HLA-DRB1*1301/1302等位基因的相关性,为寻找吸毒合并HIV感染的易感基因和抵抗基因提供线索.方法 应用序列特异性引物聚合酶链反应(polymerase chain reaction with sequencespecific primers,PCR-SSP)技术对41例无亲缘...

Relation between HLA-DRB1*1301/1302 Allele Polymorphism and HIV Infection in Drug Users

Objective To investigate the relationship between HLA-DRB1＊1301/1302 allele polymorphism and HIV infection in drug users,and provide a cue for searching the susceptible allele and resistant allele.Methods A total of 41 drug users with HIV infection and 24 drug users without genetic relationship were studied.HLA-DRB1＊1301/1302 allele polymorphism were tested by polymerase chain reaction with sequence-specific primers（PCR-SSP）,and its frequency distribution were analyzed and compared between two groups.Results The gene frequency of ＊1301/1302 allele in the control group was 0.06573.In drug user group,the frequency was 0.04167,RR=0.600649,and decreased but not significantly compared with control group（P0.05）.In HIV infected drug users,the frequency was 0.01220,RR=0.1700,and decreased significantly compared with control group（P0.05）.Compared with drug users group,there was no significant difference in the frequency between drug users and HIV infected drug users（P0.05）.Conclusion There exists close relationship between HLA-DRB1＊1301/1302 alleles and resistance of drug use with HIV infection,which plays a crucial role in the pathology,treatment and immune prevention of the disease.