重症监护病房中耐甲氧西林金葡菌的流行

目的调查耐甲氧西林金黄色葡萄球菌（ＭＲＳＡ）在重症监护病房（ＩＣＵ）的流行情况。方法将临床分离２８株ＭＲＳＡ包埋于琼脂块中，原位溶解细菌，ＳｍａⅠ消化染色体ＤＮＡ，经脉冲场凝胶电泳（ＰＦＧＥ）分离，比较染色体限制性内切酶图谱，确定菌株的亲缘关系。结果２８株ＭＲＳＡ的ＰＦＧＥ图谱有Ｋ、Ｍ、Ｌ、Ｎ４型，以Ｋ型为主（１８株）。ＩＣＵ本次流行期间的ＭＲＳＡ分离株均为Ｋ１亚型（１４株），与前次流行株Ａ１亚型完全不同。采集２０位ＩＣＵ医护人员鼻拭子，分离到２株ＭＲＳＡ，阳性率１０％，其ＰＦＧＥ图谱与流行株一致。结论ＭＲＳＡ在ＩＣＵ流行情况十分严重，及时采取有效措施控制这种流行及播散是至关重要的

Tracing the outbreak of methecilin resistant Staphylococcus aureus in intensive care units

Objective To investigate the outbreak of methicillin resistant Staphylococcus aureus(MRSA) in intensive care units.Methods Pulsed field gel electrophoresis(PFGE) was used to type the DNA of MRSA. Cells were suspended in agarose and digested in situ. The intact genomic DNA was cleaved with SmaI and electrophoresised in pulsed field.Results Four different PFGE patterns (K-N) were found among 28 MRSA strains, most of MRSA were PFGE type K (18 strains). 14 MRSA strains with PFGE type K1 isolated during the outbreak were different from the Previous outbreak strain (PFGE type A1). Nasal carriage was detected in 2 of 20(10%) health care workers in ICU, and the 2 MRSA strains had the same PFGE patterns with the outbreak strain.Conclusion Efficient infection control measures should be carried out to control MRSA outbreak and spread.