反复热性惊厥过程中气体信号分子硫化氢对一氧化氮/一氧化氮合酶体系的影响

目的：探讨硫化氢（H₂S）对热性惊厥（FS）大鼠一氧化氮（NO）/一氧化氮合酶（NOS）体系表达的影响。方法:大鼠随机分为对照组、FS组、FS+NaHS组、FS+HA（hydroxylamine）组。采用热水浴诱导大鼠FS，隔日诱导1次，共10次。采用分光光度计法测定大鼠血浆中H₂S和NO含量；用原位杂交观察nNOS mRNA表达情况；用免疫组化方法观察NOS蛋白表达情况。结果:FS+NaHS组NO含量低于FS组，同时NOS表达也低于FS组；而FS+HA组NO含量高于FS组，同时NOS表达也强于FS组。结论:用H₂S外源性供体NaHS和胱硫醚-β-合成酶抑制剂HA的干预研究表明，反复热性惊厥过程中，H₂S的改变可影响NO/NOS体系的表达。

Hydrogen sulfide regulates the expression of nitric oxide/nitric oxide synthase system during recurrent febrile seizures

AIM: To explore the effect of hydrogen sulfide (H2S) on nitric oxide (NO)/nitric oxide synthase (NOS) system during recurrent febrile seizures (FS). METHODS: Sprague-Dawley rats aged 21 days were randomly divided into four groups: control group (37.0 ℃ water, n=8); FS group (45.2 ℃ water, n=8); FS+NaHS group (45.2 ℃ water, n=8), FS+HA (hydroxylamine) group (45.2 ℃ water, n=8). FS in rats were induced ten times in a bath of warm water, once every 2 days. The plasma level of H2S and NO was detected by the spectrophotometer method. The expression of NOS mRNA was examined by in situ hybridization. The expression of nNOS protein was observed by immunohistochemistry. RESULTS: The plasma level of NO decreased significantly in FS+NaHS group while elevated obviously in FS+HA group compared with that in FS group. At the same time, the expression of nNOS down-regulated in FS+NaHS group while up-regulated in FS+HA group compared with that in FS group. CONCLUSION: H2S down-regulated the expression of NO/NOS system during recurrent FS.