| HPLC法测定复方樟脑软膏中樟脑的含量 |
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| 引用本文： | 白林,;范金钊,;徐风华,;王欢欢.HPLC法测定复方樟脑软膏中樟脑的含量[J].中国药物应用与监测,2014(4):212-213. |
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| 作者姓名： | 白林 ;范金钊 ;徐风华 ;王欢欢 |
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| 作者单位： | [1]解放军总医院药品保障中心,北京100853; [2]蚌埠医学院,安徽蚌埠233000 |
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| 基金项目： | 半固体制剂标准提高系列研究课题（13ZJZ05） |
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| 摘 要： | 目的：建立HPLC法测定复方樟脑软膏中樟脑的含量。方法：采用色谱柱Diamonsil C18柱（250 mm×4.6 mm,5μm）;流动相：甲醇-乙腈-水（15∶45∶40）;检测波长：289 nm;柱温：35℃;流速：1.0 mL·min-1;进样量：10μL。结果：樟脑与其他杂质峰分离良好,樟脑在250.00-2 000.00μg·mL-1范围内与峰面积呈良好的线性关系（r=1.000 0）,平均回收率为99.68%,RSD为1.21%（n=9）。结论：本方法简便易行、准确,可作为复方樟脑软膏中樟脑的质量控制方法。
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| 关 键 词： | 高效液相色谱法 复方樟脑软膏 樟脑 含量测定 |
Content determination of camphor in compound camphor ointment by HPLC |
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| Institution: | BAI Lin, FAN Jin-zhao, XU Feng-hua, WANG Huan-huan(1. Department of Pharmaceutical Care, PLA General Hospital, Beijing 100853, China; 2. Bengbu Medical College, Bengbu 233000, China) |
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| Abstract: | Objective： To establish the HPLC method for determining the content of camphor in compound camphor ointment. Methods：The analysis was carried out on a column of Diamonsil C18 （250 mm ＆#215; 4.6 mm, 5μm） with the mobile phase consisting of methanol-acetonitrile-water （15∶45∶40）, the flow rate was 1.0 mL＆#183;min-1, the detection wavelength was 289 nm, column temperature was 35℃, the sample injection was 10μL. Results：The samples were separated well from other substances. The calibration curve of camphor was linear in the range of 250.00-2 000.00 μg·mL^-1 （r = 1.000 0）. The average recovery of camphor in the compound camphor ointment was 99.68%with RSD of 1.21%（n=9）. Conclusion： This method is accurate and feasible, which can be applied as the quality control of camphor in the compound camphor ointment. |
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| Keywords: | HPLC Compound camphor ointment Camphor Content determination |
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