Comparison of biochemical characteristics of cultured fibrochondrocytes isolated from the inner and outer regions of human meniscus |
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Authors: | T Tanaka Katsuyuki Fujii Yoshio Kumagae |
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Institution: | (1) Department of Orthopaedic Surgery, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan Tel.: +81-3-3433-1111, ext. 3441 Fax: +81-3-3459-9114, JP |
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Abstract: | In order to evaluate the ability of fibrochondrocytes to synthesize collagen and proteoglycan, human medial meniscal cells
were cultured in a monolayer. Meniscal cells were prepared from the two regions (outer 1/3 and inner 2/3) in consideration
of the difference in vascular supply, and articular chondrocytes were also obtained from the same knee joint. Regarding total
collagen synthesis, regional differences were not found, but age-related differences were found in human medial meniscus.
In contrast, proteoglycan synthesis revealed significant regional differences; meniscal cells from the inner 2/3 synthesized
a greater amount of proteoglycan. After long-term monolayer culturing, proteoglycan synthesis by meniscal cells decreased
in a time-dependent manner, and morphological changes to fibroblast-like cells were found. In the presence of transforming
growth factor (TGF)-β, proteoglycan synthesis increased in a dose-dependent manner. These findings suggest that the inner
regions of the human meniscus contain cells with a chondrocytic phenotype.
Received: 11 February 1998 Accepted: 14 September 1998 |
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Keywords: | Meniscus Fibrochondrocytes Fibrocartilage Proteoglycan Collagen |
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