去氢吴茱萸碱及盐酸小檗碱对D-半乳糖致衰老模型小鼠学习记忆的影响

对去氢吴茱萸碱(DHED)及盐酸小檗碱(BH)对D-半乳糖致衰老模型小鼠学习记忆能力及抗氧化能力的影响进行了研究，并初步探讨了作用机制。通过D-半乳糖(120 mg/kg)颈背部皮下注射给药60 d造成亚急性衰老模型，并于造模开始10 d前灌胃给药，连续给药70 d。采用Morris水迷宫仪测定小鼠学习记忆情况，并测定脑组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。结果显示，在定向航行试验中，模型组逃避潜伏期及搜索距离均明显高于正常对照组(P<0.05，P<0.05)；与模型组相比，DHED高剂量组逃避潜伏期及搜索距离显著缩短(P<0.05，P<0.05)；空间探索实验发现，模型组在原平台象限探索时间百分比明显低于正常对照组(P<0.01)；DHED高、低剂量组和BH低剂量组在原平台象限探索时间百分比，探索距离百分比明显高于模型组(P<0.001，P<0.05，P<0.05)。酶活性检测发现，与正常对照组相比，模型组小鼠脑内SOD活性明显降低(P<0.05)；与模型组相比，DHED高剂量组和BH低剂量组脑内SOD活性明显提高(P<0.01，P<0.05)。实验结果表明：DHED及BH能够改善D-半乳糖诱导的衰老小鼠模型的学习记忆功能障碍，且前者具有更明显的改善效果，该作用可能与抗氧化应激有关。

Effects of dehydroevodiamine and berberine hydrochloride on learning and memory in aging model mice induced by D-galactose

The study was to explore the effects of dehydroevodiamine(DHED) and berberine hydrochloride(BH) on learning and memory and anti-oxidative capability in aging model mice induced by D-galactose，and to approach the molecular mechanisms of action.D-galactose(120 mg/kg) was sc injected into mice for 60 days to establish the subacute aging model，and medication groups were ig administered 10 days before animal modeling.Morris water maze testing apparatus was used to determine the ability of mice′s learning and memory；the activity of SOD and the contents of MDA in the brain tissue were detected separately.The results showed that in place navigation test，the escape latency and searching distance of model mice were significantly longer than those of the control group(P<0.05，P<0.05)；and DHED high-dose-treated group had shorter escape latency and searching distance compared with the model group.In space probe test，the control group had longer staying time in original platform quadrant than the model group(P<0.01).DHED-treated groups and BH low dose group could significantly prolong the staying time and distance of the original platform quadrant(P<0.001，P<0.05，P<0.05).Compared with the control group，the SOD activity in the brain of model mice decreased(P<0.05)；the SOD activity of brain were enhanced significantly in high-dose DHED group and low-dose BH group in comparison with the model group(P<0.01，P<0.05).These findings indicate that DHED and BH could improve the ability of learning and memory of model mice induced by D-galactose，and that the former has better effect with its mechanism being related with the ability of anti-oxidative stress.