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| 浙江省家畜动物无形体分子流行病学调查 | |
| 引用本文： | 陆群英,张立霞,孙继民,姜理平,柴程良,郑寿贵,顾时平,葛君华,王誓闻,禹惠兰,张丽娟. 浙江省家畜动物无形体分子流行病学调查[J]. 中国媒介生物学及控制杂志, 2011, 22(3): 226-228,232 |
| 作者姓名： | 陆群英 张立霞 孙继民 姜理平 柴程良 郑寿贵 顾时平 葛君华 王誓闻 禹惠兰 张丽娟 |
| 作者单位： | 1. 浙江省疾病预防控制中心微生物所,杭州,310051 2. 中国疾病预防控制中心传染病预防控制所,北京,102206 3. 金华市金东区疾病预防控制中心 4. 安吉县疾病预防控制中心 5. 天台县疾病预防控制中心 |
| 基金项目： | 国家基础研究项目973计划,重大传染病防治科技专项,％％ |
| 摘 要： | 目的调查浙江省天台、安吉及金华县家畜动物牛及山羊无形体感染状况。方法以在册户籍为准,采用随机方法抽取调查点每农户5只山羊及5头牛,采集5ml静脉血分离血清,采用WHO推荐的微量间接免疫荧光方法（mIFA）进行血清IgG抗体检测;动物血球标本用于提取DNA进行无形体16SrRNA基因扩增。PCR产物测序并进行同源分析比较,同时进行遗传变异分析。结果当地家畜动物存在较高无形体携带率（39.42%）,主要分布在天台县;无形体流行株16SrRNA基因存在较大变异性,除发现15种独立核酸变异序列外,至少存在5种优势序列变异株。结论浙江省家畜动物普遍存在无形体携带状况;进一步开展人群无形体病调查及分子流行病学研究十分必要。 |
| 关 键 词： | 无形体 遗传变异 家畜动物 浙江省 |
Molecular epidemiological survey of Anaplasma phagtocytophilium in livestock in Zhejiang province |
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| LU Qun-ying,ZHANG Li-xia,SUN Ji-min,JIANG Li-ping,CHAI Cheng-Hang,ZHENG Shou-gui,GU Shi-ping,GE Jun-hua,WANG Shi-wen,YU Hui-lan,ZHANG Li-juan. Molecular epidemiological survey of Anaplasma phagtocytophilium in livestock in Zhejiang province[J]. Chinese Journal of Vector Biology and Control, 2011, 22(3): 226-228,232 | |
| Authors: | LU Qun-ying ZHANG Li-xia SUN Ji-min JIANG Li-ping CHAI Cheng-Hang ZHENG Shou-gui GU Shi-ping GE Jun-hua WANG Shi-wen YU Hui-lan ZHANG Li-juan |
| Affiliation: | LU Qun-ying1,ZHANG Li-xia2,SUN Ji-min1,JIANG Li-ping1,CHAI Cheng-liang1,ZHENG Shou-gui3,GU Shi-ping4,GE Jun-hua5,WANG Shi-wen2,YU Hui-lan2,ZHANG Li-juan2 1 Zhejiang Center for Disease Control and Prevention,Hangzhou 310051,Zhejiang Province,China,2 National Institute of Communicable Disease Control and Prevention,China CDC,Beijing 102206,3 Jindong District Center for Disease Control and Prevention,Jinhua,4 Anji Center for Disease Control and Prevention,5 Tiantai Center for Disease Control and Preventi... |
| Abstract: | Objective To determine the prevalence of Anaplasma phagocytophilum in goats and oxen in Zhejiang province. Methods Five goats and five oxen were randomly surveyed in each househould based on registration information. Venous blood samples of 5 ml were collected from each subject,and the separated serum was tested for IgG antibodies against A. phagocytophilum using immunofluorescence assay(mIFA) as recommended by the WHO. The blood cells were used for DNA extraction,which was then amplified for the 16S rRNA g... |
| Keywords: | Anaplasma phagocytophilum Genetic variation Domestic animals Zhejiang province |
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