| 两个地理株青海血蜱基因组DNA差异研究 |
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| 引用本文： | 史智勇,赵红斌,杨银书,常德辉,张继军. 两个地理株青海血蜱基因组DNA差异研究[J]. 中华卫生杀虫药械, 2004, 10(5): 310-312 |
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| 作者姓名： | 史智勇 赵红斌 杨银书 常德辉 张继军 |
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| 作者单位： | 1. 兰州军区总医院,甘肃,兰州,730050
2. 兰州军区军事医学研究所,甘肃,兰州,730020 |
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| 摘 要： | 目的研究青海血蜱是否有种型差异.方法分别提取青海血蜱两个地理株的DNA,选取8条不同的随机排列碱基顺序的多聚核苷酸单链为引物,进行RAPD-PCR扩增反应.将扩增产物制备成DNA图谱,比较青海血蜱两个地理株基因组DNA多态性.从DNA水平对青海血蜱两个地理株进行差异分析.结果青海血蜱两个地理株大部分DNA条带是相同的,少数DNA条带不同.结论两个地理株青海血蜱DNA存在着差异.
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| 关 键 词： | 青海血蜱 地理株 |
| 文章编号： | 1671-2781(2004)05-310 |
| 修稿时间： | 2004-04-12 |
Studies on differences of DNA of two strains of Haemaphysalis qinghaiensis |
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| SHI Zhi-yong,ZHAO Hong-bin,YANG Yin-shu,CHANG De-hui,ZHANG JI-Jun. Studies on differences of DNA of two strains of Haemaphysalis qinghaiensis[J]. Chinese Journal of Hygienic Insecticides and Equipments, 2004, 10(5): 310-312 |
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| Authors: | SHI Zhi-yong ZHAO Hong-bin YANG Yin-shu CHANG De-hui ZHANG JI-Jun |
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| Abstract: | Objective To study whether two strains of Haemaphysalis qinghaiensis had genomic DNA differences. Methods The genomic DNA was extracted respectively from two strains of Haemaphysalis qinghaiensis,8 primers with different arbitrary single chain polynucleotide sequences were selected,the genomic DNA was amplified by RAPD-PCR.DNA fingerprint maps were made with the amplified products.The polymorphism DNA of the two strains of Haemaphysalis qinghaiensis was compared,and their diversities were analyzed on DNA level. Results Major DNA fragments between two strains were the same,minor DNA fragments between them were different. Conclusion The diversities of genomic DNA between two strains of Haemaphysalis qinghaiensis existed. |
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| Keywords: | RAPD |
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