杜仲带腋芽茎段组织培养的研究

目的研究不同培养条件下杜仲带腋芽茎段愈伤组织的诱导和生长情况,以及腋芽的生长情况。方法以带腋芽的杜仲茎段为外植体,设计9种不同的培养基配方,为了防止褐化,在23.4℃下培养箱中暗培养,30 d后调查出愈率及腋芽的生长情况。结果 NAA浓度从0.05~0.5 mg.L-1,随着浓度增加,腋芽生长受抑制;6-BA浓度从0.3~1.0 mg.L-1,随着浓度增加,愈伤组织块增大,达到1.0 mg.L-1时愈伤组织的生长受到抑制;本实验中最佳的杜仲愈伤组织诱导和生长培养基配方为6号培养基:B5+0.5 mg.L-1NAA+0.3 mg.L-16-BA。腋芽生长最佳培养基配方为4号培养基:B5+0.05 mg.L-1NAA+0.5 mg.L-16-BA。结论本试验的结果对杜仲的生物技术利用有一定的参考价值。

Tissue culture of Eucommia ulmoides Oliv. stem fragments with axillary buds

Objective To study the callus induction and growth condition of Eucommia ulmoides Oliv.stem fragments with axillary buds under different culture conditions,and observe the growth status of axillary buds.Methods Eucommia ulmoides Oliv.stem fragments with axillary buds were used as explants.The stems were cultured under 9 culture media at 23.4 ℃ in dark to avoid browning.We observed the rate of callus and the growth status after 30 days.Results The growth status of axillary buds was inhibited by naphthylacetate（NAA） within 0.05-0.5 mg·L-1.The callus increased with 6-benzyladenine（6-BA） increased from 0.3 to 1.0 mg·L-1.When the conrentration increased to 1.0 mg·L-1,the growth was inhibited conversely.The best induction and growtn culture medium for callus was B5＋0.5 mg·L-1NAA＋0.3 mg·L-16-BA,and the best growth culture medium for axillary buds was B5＋0.05 mg·L-1NAA＋0.5 mg·L-16-BA.Conclusion The study provides a reference for the biotechnological of Eucommia ulmoides Oliv.