黏蛋白-1和细胞角蛋白-20在食管癌中的表达及意义

 目的　研究食管癌患者外周血黏蛋白-1（MUC1）和细胞角蛋白-20（CK20）表达情况及临床意义。方法　采用反转录聚合酶链反应（RT-PCR）方法检测53例食管癌患者外周血MUC1-mRNA和CK20-mRNA水平，以10例食管良性肿瘤患者及20名健康体检者为对照。结果　在良性肿瘤组及健康对照组中，MUC1-mRNA及CK20-mRNA均无表达，而在食管癌患者中的阳性率分别是35.85 %（19／53）和49.06 %（26／53），二者联合检测阳性率为62.26 %（36／53），较单项阳性率高（χ2＝11.0228，P＜0.05）。二者在食管癌患者中的表达与患者性别、年龄及肿瘤分化程度无明显相关性，而与肿瘤临床分期明显相关。结论　MUC1-mRNA和CK20-mRNA作为食管癌肿瘤标志物具有较高的敏感性及特异性；其表达与食管癌的临床分期密切相关；两种标志物联合检测有助于提高食管癌微转移的检出率。

Expressions and clinical significances of MUC1-mRNA and CK20-mRNA in esophageal cancer

Objective To investigate the expressions and clinical significances of MUC1-mRNA and CK20-mRNA in peripheral blood of esophageal cancer patients.Methods MUC1-mRNA and CK20-mRNA were detected in 53 patients with esophageal cancer,10 patients with esophageal benign tumor and 20 healthy volunteers by RT-PCR technique.Results The expressions of MUC1-mRNA,CK20-mRNA and combining group were 35.85 % (19/53),49.06 % (26/53) and 62.26 % (36/53) in peripheral blood of 53 esophageal cancer patients.In control group,there was no expression of MUC1-mRNA and CK20-mRNA in peripheral blood of 10 patients with benign esophageal disease and 20 healthy volunteers.The positive rate increased by combining test(x2 =11.0228,P ＜0.05).Conclusion MUC1-mRNA and CK20-mRNA might be specific and sensitive markers to detect circulating tumor cells in peripheral blood and their expressions are closely related to TNM stages of the esophageal cancer patients.The combining test might be of high value of the diagnosis of micrometastasis.