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| 反相高效液相色谱法测定注射用苯唑西林钠有关物质 | |
| 引用本文： | 吴琦琦,刘雁鸣,倪孟祥. 反相高效液相色谱法测定注射用苯唑西林钠有关物质[J]. 中南药学, 2011, 9(3): 203-205. DOI: 10.3969/j.issn.1672-2981.2011.03.014 |
| 作者姓名： | 吴琦琦 刘雁鸣 倪孟祥 |
| 作者单位： | 1. 湖南省药品检验所,长沙,410001;中国药科大学,南京,210009 2. 湖南省药品检验所,长沙,410001 3. 中国药科大学,南京,210009 |
| 摘 要： | 目的建立RP-HPLC法测定苯唑西林钠的有关物质。方法采用C18色谱柱;流动相:0.01 mol.L-1醋酸铵溶液(醋酸调pH至5.0)-乙腈(75∶25);流速:1.0 mL.min-1;检测波长:225 nm;柱温:30℃。结果苯唑西林钠线性范围为1.171~28.11μg.mL-1,r=0.999 8,检测限:0.036μg.mL-1,苯唑西林钠能与各杂质完全分离。结论建立的苯唑西林钠有关物质的测定方法简单、快速、重现性好。 |
| 关 键 词： | 反相高效液相色谱法 苯唑西林钠 有关物质 |
Determination of relavant substances of oxacillin sodium for injection by RP-HPLC |
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| WU Qi-qi,LIU Yan-ming,NI Meng-xiang. Determination of relavant substances of oxacillin sodium for injection by RP-HPLC[J]. Central South Pharmacy, 2011, 9(3): 203-205. DOI: 10.3969/j.issn.1672-2981.2011.03.014 | |
| Authors: | WU Qi-qi LIU Yan-ming NI Meng-xiang |
| Affiliation: | WU Qi-qi1,2,LIU Yan-ming1*,NI Meng-xiang2(1.Hunan Institute for Drug Control,Changsha 410001,2.China Pharmaceutical University,Nanjing 210009) |
| Abstract: | Objective To establish an RP-HPLC method for the determination of relavant substance of oxacillin sodium for injection.Methods The chromatographic analysis was performed on C18 column with 0.01 mol·L-1ammonium acetate（pH 5.0）-acetonitrile（75∶25） as the mobile phase,the temperature was 30℃,flow rate was 1.0 mL·min-1,and detection wavelength was 225 nm.Results The linear range was 1.171-28.114 μg·mL-1（r=0.999 8）,and the detection limit was 0.036 μg·mL-1.The relavant substances were separated from oxacillin sodium.Conclusion The method is rapid,simple and accurate to determine the relavant substances of oxacillin sodium. |
| Keywords: | RP-HPLC oxacillin sodium relavant substance |
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