高效液相色谱法测定灯盏细辛胶囊中焦袂康酸的含量

目的建立高效液相色谱法测定灯盏细辛胶囊中焦袂康酸含量的方法。方法采用高效液相色谱法,色谱柱:Agilent C18(TC)(250 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸溶液,梯度洗脱;流速:0.8 mL.min-1;检测波长:270 nm;柱温:35℃。结果焦袂康酸进样量在0.010 176~0.848μg与峰面积线性关系良好,r=0.999 0,平均加样回收率为103.3%,RSD=0.34%。结论所建立的含量测定方法简便可行,结果准确可靠,可用于灯盏细辛胶囊中焦袂康酸的含量测定。

Determination of pyromeconic acid in Dengzhan Xixin Jiaonang by HPLC

Objective To establish an HPLC method for the determination of pyromeconic acid in Dengzhan Xixin Jiaonang.Methods HPLC method was used with Agilent C18（TC） column（250 mm ×4.6 mm,5 μm）.The mobile phase consisted of methanol and 0.1% H3PO4 solution with gradient elution,and the flow rate was 0.8 mL·min-1.The detective wavelength was 270 nm,and the column temperature was 35 ℃.Results The linear range of pyromeconic acid was 0.010 176-0.848 μg.The average recovery of pyromeconic acid was 103.3% with RSD=0.34%.Conclusion The method is accurate and can be used for the content determination of pyromeconic acid in Dengzhan Xixin Jiaonang.