|
|||||
|
|
| 熊果酸对人胃癌细胞株MGC-803增殖的影响及机制 | |
| 引用本文： | 刘蔷,向谨逸,桂军宏.熊果酸对人胃癌细胞株MGC-803增殖的影响及机制[J].实用医学杂志,2007,23(21):3318-3320. |
| 作者姓名： | 刘蔷 向谨逸 桂军宏 |
| 作者单位： | 1. 湖北省新华医院中医科,武汉市,430015 2. 华中科技大学同济医学院生物化学系,武汉市,430030 3. 华中科技大学同济医学院附属同济医院中医科,武汉市,430030 |
| 摘 要： | 目的 观察熊果酸(Ursolic acid, UA)对胃癌细胞株(MGC-803)的抑制增殖作用。方法 培养胃癌细胞株(MGC-803)，以MTT比色法及克隆形成实验测定UA对胃癌细胞株MGC-803抑制增殖的作用。免疫印记法测定p-ERK1/2、Cyclin D1及p21waf/cip1表达。结果 MTT实验及克隆分析法均显示UA明显抑制MGC-803细胞增殖，免疫印记法显示UA抑制p-ERK1/2、Cyclin D1表达，同时上调p21waf/cip1表达。结论 熊果酸可以抑制胃癌细胞株(MGC-803)增殖，机制与影响p-ERK1/2及下游Cyclin D1、p21waf/cip1表达有关。 |
| 关 键 词： | 熊果酸 胃癌 增殖 |
| 收稿时间： | 2007-5-14 |
| 修稿时间： | 2007-05-12 |
Effects of Ursolic acid on the proliferation of human gastric carcinoma MGC-803 cell line |
|
| LIU Qiang,XIANG Jin-yi,GUI Jun-hong.Effects of Ursolic acid on the proliferation of human gastric carcinoma MGC-803 cell line[J].The Journal of Practical Medicine,2007,23(21):3318-3320. | |
| Authors: | LIU Qiang XIANG Jin-yi GUI Jun-hong |
| Institution: | Department of Traditional Chinese Medicine, Xinhua Hospital, Wuhan 430015, China |
| Abstract: | Objective To observe the effects of ursolic acid (UA) on the proliferation of human gastric carcinoma cell line MGC-803. Methods Human gastric carcinoma cell line MGC-803 was cultured and the effects of UA on the proliferation of MGC-803 cells were determined by MTT assay and clone formation experiment. The expressions of p-ERK1/2,Cyclin D1,and p21waf/cip1 were detected by Western blot. Results The proliferation of MGC-803 cells was obviously suppressed. The expressions of p-ERK1/2 and cyclin D1 were inhibited whereas p21waf/cip1 expression was upregulated by UA. Conclusion UA can suppress the proliferation of gastric carcinoma cell line MGC-803, which is involved in the regulation of p-ERK1/2, cyclin D1, and p21waf/cip1. |
| Keywords: | Stomach neoplasms Ursolic acid Proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《实用医学杂志》浏览原始摘要信息 | |
| 点击此处可从《实用医学杂志》下载免费的PDF全文 | |