梅毒患者外周血单一核细胞诱导的树突细胞的生物学特性

目的 探讨梅毒患者外周血单一核细胞（PBMC）诱导的树突细胞（DC）的生物学特性。方法 分离诱导产生单一核细胞来源的树突细胞（MoDC），比较梅毒患者与正常对照组MoDC表型的差异；用重组梅毒螺旋体脂蛋白TpN17体外刺激正常人MoDC后观察其表型的变化、免疫印迹法检测细胞外信号调节激酶（ERK）磷酸化的情况。结果 与正常对照组相比，梅毒患者MoDC细胞表面CD80（51.90%）表达明显上升（P < 0.05）。CD83（16.53%）、CD86（66.13%）及HLA-DR（91.29%）的表达率与对照组差异无统计学意义（P > 0.05）。TpN17可以刺激MoDC表面CD80、CD83表达升高，细胞表达磷酸化ERK1/2（p-44/42）；未经TpN17刺激的DC，无磷酸化ERK蛋白表达。结论 梅毒患者MoDC表型的异常可能与梅毒螺旋体抗原刺激有关，TpN17可以刺激DC成熟，其信号转导途径与ERK有关。

Biological characteristics of dendritic cells derived from peripheral blood of patients diagnosed with syphilis

Objective To study the biological characteristics of dendritic cells (DCs) derived from peripheral blood mononuclear cells (PBMCs) of patients diagnosed with syphilis. Methods PBMCs were isolated from 16 patients clinically and serologically diagnosed with syphilis, and from 16 healthy human controls, then cultured with GM-CSF and IL-4. On day 10, the monocyte-derived dendritic cells (MoDCs)of the patients and controls were collected and subjected to the detection of surface molecules by flow cytometry; TpN17 was used to stimulate MoDCs from the controls, the expression of phosphorylated ERK was detected by Westem blotting 20 minutes following the stimulation. Results The positivity rate of CD80 was significantly increased in the patients with syphilis than that in the controls (51.90% vs 33.67,P ＜ 0.05), while no significant difference was observed in the expressions of CD83, CD86 or HLA-DR be tween the two groups (16.53% vs 15.99%, 66.13% vs 59.32%, 91.29% vs 90.51%, all P 0.05). The ex pressions of CD80 and CD83 on the surface of MoDCs were enhanced in a dose-dependent manner after ex-posure to TpN17. The expression of cytoplasmic phosphorylated ERK was observed in MoDC stimulated by TpN17, but not in those without the treatment. Conclusions Antigenic stimulation with Treponema pal-lidum may be a reason for phenotypic abnormality of MoDCs derived from patients with syphilis. TpN17 may stimulate the maturation of DCs through the ERK signal transduction pathway.