adenoviral-mediated hath1-egfp gene transfer into guinea pig cochlea through intact round window membrane |
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作者单位: | [1]neurologic biological centre, institute of otolaryngology, department of otorhinolaryngology-head & neck surgery, chinese pla general hospital, beijing 100853, china; [2]department of biomedical sciences, creighton university, 2500 california plaza, omaha, ne 68175, usa |
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摘 要: |
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收稿时间: | 4 May 2008 |
Adenoviral-mediated Hath1-EGFP gene transfer into guinea pig cochlea through intact round window membrane |
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Authors: | CHEN Wei HU Yin-yan YANG Shi-ming GUO Wei SUN Jian-he HAN Dong-yi ZHAI Suo-qiang YANG Wei-yan David ZZHe |
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Institution: | 1. Neurologic Biological centre, Institute of Otolaryngology, Department of Otorhinolaryngology–Head & Neck Surgery, Chinese PLA General Hospital, Beijing 100853, China;2. Department of Biomedical Sciences, Creighton University, 2500 California Plaza, Omaha, NE 68175, USA |
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Abstract: | Objective To study expression of adenovira1-mediated Hathl-EGFP gene in the guinea pig cochlea after transfer through intact round window membrane (RWM), and to assess its effects on hearing. Methods Twenty adult guinea pigs were used, of which: 12 were surgically inoculated with AdHath1-EGFP in the bony groove of round window niche, and 8 with artificial perilymph. Auditory brainstem response(ABR) thresholds were determined in all animals before and 5 days after surgery. On post-surgery day 5 and day 14, animals were sacrificed and whole mounts of cochlea and fro zensections were examined. Results ABR tests showed no significant change of hearing after the surgery.Strong fluorescence staining in the cochleae was seen in Ad-Hathl-EGFP groups. The highest levels of gene expression were seen in the post-surgery day 5 group with tittle decrease on post-surgery day 14.The contralateral cochlea and those in the control groups were free of fluorescence staining. Conclusion The transgenic Hath1-EGFP can be effectively delivered into the inner ear through intact RWM, in an atraumatic manner. |
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Keywords: | Gene transfer round window membrane Adenovirus guinea pig Hath1 |
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