实时定量RT-PCR检测乳腺癌组织Drosha基因表达及其意义

目的通过检测Drosha在乳腺癌组织中的表达情况,探讨其在乳腺癌发生发展中的意义。方法乳腺癌肿瘤组织与正常乳腺组织标本各78例自身配对分成肿瘤组与正常组,提取总RNA,利用SYBR Green1实时定量RT-PCR方法检测Drosha基因mRNA的表达情况。结果在mRNA水平上,Drosha基因在71.80%(56/78)的癌组织中下调,28.20%(22/78)上调。经REST软件分析,其表达下调具有统计学意义(P0.01),Drosha mRNA低表达与TNM分期差异有统计学意义(P0.01),与患者年龄、月经状态、肿瘤大小、淋巴结是否转移、组织学分级、激素受体状态(ER、PR和HER-2)等差异均无统计学意义(P0.05)。结论 Drosha基因在乳腺癌中表达下调,可能与乳腺癌的发生发展有关。

Real-time Quantitative RT-PCR Detection of Drosha Gene Expression in Breast Cancer and Its Clinical Significance

Objective To detect the Drosha expression in breast cancer and explore its clinical significance. Methods Seven-eight paired samples of breast cancer and normal breast tissue were allocated into caner group and normal group. The extraction of total RNA was carried out and the mRNA expression of Drosha gene was detected by using relative Quantitative Real-Time RT-PCR. Results At the mRNA level, Drosha gene expression showed the down-regulation in 71.80%（56/78 ） cancer tissue and the up-regulation in 28.20% （22/78） cancer tissue, and the mRNA expression level in control group was higher than that in cancer group（P〈0.01 ）. the mRNA expression level of Dicer gene in human breast cancer was correlated with TNM staging（P〈0.01 ）, but had no significant correlation with age, menopausal status, tumor size, lymph node metastasis and hormone receptor status（P〉0.05 ）. Conclusion The expression level of Drosha gene is down-regulated in breast cancer. Drosha may play an important role in the occurrence of breast cancer.