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| 反义封闭PKARⅠβ基因表达对双龙接骨丸含药血清体外促成骨细胞增殖作用的影响 | |
| 引用本文： | 孙奋勇,潘秋辉,洪岸. 反义封闭PKARⅠβ基因表达对双龙接骨丸含药血清体外促成骨细胞增殖作用的影响[J]. 中国病理生理杂志, 2004, 20(12): 2316-2319 |
| 作者姓名： | 孙奋勇 潘秋辉 洪岸 |
| 作者单位： | 1. 暨南大学生物工程研究所, 广东 广州 510632; 2. 中山大学附属孙逸仙纪念医院医学研究中心, 广东 广州 510120 |
| 摘 要： | 目的:进一步明确cAMP依赖的磷酸激酶Ⅰ型调节亚基β(PKARⅠβ)在双龙接骨丸含药血清促进体外培养成骨细胞增殖过程中的作用。方法:构建表达 PKARⅠβ基因反义序列的重组子pcDNA-antiPKARⅠβ,脂质体法转导成骨细胞HFOB1.19,MTT法检测其增殖状况。结果:高剂量含药血清处理的反义基因封闭组中,HFOB1.19的增殖能力与空白血清对照组无显著差异(P>0.05)。结论:双龙接骨丸含药血清的体外促成骨细胞增殖作用与PKARⅠβ基因的表达密切相关。 |
| 关 键 词： | 双龙接骨丸 成骨细胞 基因表达 核酸 反义 |
| 文章编号： | 1000-4718(2004)12-2316-04 |
| 收稿时间： | 2003-12-16 |
Effects of blocking the expression of PKARⅠβ gene with antisense nucleotide on Shuanglong-Jiegu pill (SLJGP) containing serum-induced osteoblast proliferation |
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| SUN Fen-yong,PAN Qiu-hui,HONG An. Effects of blocking the expression of PKARⅠβ gene with antisense nucleotide on Shuanglong-Jiegu pill (SLJGP) containing serum-induced osteoblast proliferation[J]. Chinese Journal of Pathophysiology, 2004, 20(12): 2316-2319 | |
| Authors: | SUN Fen-yong PAN Qiu-hui HONG An |
| Affiliation: | 1. Bioengineering Institute of Jinan University, Guangzhou 510632,China; 2. Medical Research Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China |
| Abstract: | AIM: To further investigate the role of PKARⅠβ in the growth-promoting effects of shuang long Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA- antiPKARⅠβ, a recombinant expressing the antisense sequence of PKARⅠβ, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A. |
| Keywords: | Shuanglong-Jiegu pill Osteoblasts Gene expression Nucleotide antisense |
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