肝细胞生长因子对星形胶质细胞缺氧缺糖损伤的影响

目的：研究肝细胞生长因子(HGF)对缺氧、缺糖诱导的星形胶质细胞（As）损伤及凋亡的影响。方法：以缺氧、缺糖来诱导原代培养的大鼠大脑皮层星形胶质细胞损伤。用不同终浓度（20～100ng/mL）的HGF处理星形胶质细胞，以乳酸脱氢酶（LDH）漏出率作为细胞损伤指标，四甲基偶氮唑盐（MTT）还原试验检测细胞活力，流式细胞术检测凋亡细胞，透射电镜观察细胞超微结构。结果：缺氧缺糖引起LDH漏出率增高，细胞活力下降，细胞凋亡率增高。HGF处理后，LDH漏出率降低，细胞活力增高，同时细胞凋亡率降低（P＜0.05），最大效应剂量为60ng/mL。结论： HGF对星形胶质细胞缺氧缺糖损伤具有保护作用，能减少星形胶质细胞凋亡，并呈一定的剂量依赖关系。

Effect of hepatocyte growth factor on oxygen-glucose deprived injury of astrocytes

OBJECTIVE: To explore the effect of hepatocyte growth factor (HGF) on oxygen-glucose deprived injury and apoptosis of astrocytes. METHODS: The injury of primary cultured rat cerebral cortical astrocytes was induced by oxygen-glucose deprivation. Astrocytes were treated with HGF at various final concentrations of 20 - 100 ng/mL. The cell damage and viability were evaluated by the lactate dehydrogenase (LDH) released rate and the 3- (4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion method. Detection of apoptotic cells was determined by the flow cytometry, and the ultrastructure was observed by the transmission electron microscope. RESULTS: Oxygen-glucose deprivation increased the LDH release rate, decreased the cell viability and increased the number of apoptotic astrocytes. While exposed to HGF at the same condition, the LDH release rate decreased, the cell viability increased, and the percentage of apoptotic cells decreased (P <0.05). The maximum protective effect of HGF was observed at 60 ng/mL. CONCLUSION: HGF can protect cultured astrocytes from oxygen-glucose deprived injury, and attenuate the apoptosis of astrocytes in a dose-dependent manner.