c-Myc和端粒酶在幽门螺杆菌相关性胃病中协同表达

目的 通过检测幽门螺杆菌(Hp)相关性胃病慢性浅表性胃炎(CSG)、慢性萎缩性胃炎(CAG)、异型增生及胃癌]中c—Myc和端粒酶的活性,分析c—Myc和端粒酶两者表达的关系,并探讨其与胃癌危险因子Hp之间的关系。方法 117例组织标本中c-Myc蛋白表达采用免疫组化法检测,端粒酶活性采用端粒酶PCR-ELISA法检测。结果 胃癌组中,端粒酶阳性率和c-Myc表达率(分别为87．7％和61．5％)高于非癌组;CAG伴有中、重度肠化组,端粒酶阳性率和c-Myc表达率(分别为52．4％和47．6％)显著高于CAG伴轻度肠化组(分别为13．3％和16．7％);有中、重度肠化的CAG组,伴有Hp感染者其端粒酶阳性率和c—Myc表达率(分别为67．9％和67．9％)显著高于无Hp感染者(分别为21．4％和7．1％);CSG组中无端粒酶阳性者。在胃癌及有中、重度肠化的CAG组,伴有Hp感染者其端粒酶阳性率和c—Myc表达率呈协同性增高(协同表达率分别为89．5％和100．0％)。结论 Hp感染可诱导慢性胃病、尤其是CAG中c-Myc和端粒酶的表达。在胃癌及CAG伴中、重度肠化的患者c-Myc和端粒酶协同表达。

Coordinated expression of c-Myc and telomerase activity in Helicobacter pylori infected gastric diseases

Objective To detect the telomerase activity and c Myc expression in gastric diseases and to examine the relation between these values and Helicobacter pylori (Hp)as a risk factor for gastric cancer Methods 171 gastric samples were studied to detect telomerase activity with telomerse polymerase chain reaction enzyme linked immunosorbent assay and c Myc expression using immunohistochemistry Results Telomerase activity and c Myc expression were higher in cancers (87 7% and 61 5%) than in noncancerous tissues Telomerse activity and c Myc expression were higher in chronic atrophic gastritis with moderate or severe intestinal metaplasia (52 4% and 47 6%) than in chronic atrophic gastritis with mild intestinal metaplasia (13 3% and 16 7%) In chronic atrophic gastritis with moderate or severe intestinal metaplasia, telomerse activity and c Myc expression were higher in cases with Hp infection (67 9% and 67 9%) than in these without infection (21 4% and 7 1%) c Myc epression was higher in gastric cancer with Hp infection (77 3%) than that without infection (28 6%) Telomerase activity and c Myc expression were coordinately up regulated in Hp infected gastric cancer and chronic atrophic gastritis with moderate or severe intestinal metaplasia Conclusions Hp infection may influence both c Myc expression and telomerase activity in gastric diseases, especially in chronic atrophic gastritis c Myc and telomerase co expressed in gastric cancer and chronic atrophic gastritis with moderate or severe intestinal metaplasia