三氧化二砷对成纤维细胞增殖及分泌功能的影响

目的观察三氧化二砷(As2O3)对体外培养的3T3成纤维细胞的增殖及分泌功能的影响。方法体外培养3T3成纤维细胞,用As2O3浓度分别为0(DMEM对照组)、2μmol/L和4μmol/L的DMEM培养液分别作用于成纤维细胞,24h和48h后MTT法观察细胞生长抑制情况,用细胞抑制率表示;As2O3各组作用细胞48h后Western blot检测I型胶原(ColI)的表达;As2O3各组作用细胞48h后放射免疫法检测上清液白细胞介素(IL)-6、IL-8的水平。结果 As2O3可显著抑制3T3成纤维细胞增殖,且呈剂量-效应关系(P0.01)。As2O3组Ⅰ型胶原的量显著低于DMEM对照组(P0.01),但4μmol/LAs2O3组与2μmol/LAs2O3组相比无显著性差异(P0.05)。As2O3组IL-6和IL-8水平均显著低于DMEM对照组(P0.01),但2μmol/L和4μmol/LAs2O3组相比差异无显著性(P0.05)。结论 As2O3抑制3T3成纤维细胞增殖可能与抑制CoII、IL-6及IL-8的表达有关。

Inhibitory effect of arsenic trioxide on the proliferation of 3T3 fibroblast and the expression of collagen and cytokines

Objective To observe the effect of arsenic trioxide （As2O3） on proliferation and the excretory function of 3T3 fibroblasts. Methods 3T3 fibroblasts were cultured in DMEM medium as control group or medium supplemented with 2μ mol/L, 4μ mol/L of As2O3 for 24h and 48h respectively. MTT assay was carried out to study cell proliferation; collagen type I （Col I） was determined with Western blot; The concentrations of interleukin （IL） -6 , IL-8 were observed by radioimmunoassay . Results As2O3 treatment significantly inhibited fibroblast proliferation in a time-and dosedependent manner （P〈0.01）. Col I protein expression level was significantly lower in two treated group than in DMEM control group（P〈0.01）, but with no significant difference （P〉0.05） between two treated groups . The levels of IL-6 and IL-8 were significantly lower in the treated groups than in DMEM control （P〈0.01）, but with no significant difference（P〉0.05） between two treated groups. Conclusion The inhibition effect of As2O3 on 3T3 fibroblast proliferation might be related to the downexpression of ColI, IL-6 and IL-8.