密蒙花提取物治疗兔去势所致干眼症

目的观察密蒙花提取物对去势所致的干眼症雄免基础泪液分泌量，泪腺局部炎症因子转化生长因子β1（transforming growth factor-β1，TGF-β1）、白细胞介素1β（interleukin-1β，IL-1β）、肿瘤坏死因子α（tumor necrosis factor—α，TNF—α）的影响，探讨密蒙花提取物抗雄激素水平下降所致的干眼症的作用机制。方法将30只日本大耳雄兔随机分为空白组（A）、模型组（B）、1倍剂量密蒙花提取物治疗组（C）、2倍剂量密蒙花提取物治疗组（D）和染料木黄酮治疗组（E），每组6只，对B、C、D、E组行雄激素去势术建立动物模型，对C、D、E组分别以密蒙花提取物1倍剂量和2倍剂量以及染料木黄酮连续灌胃1个月，对全部实验兔行Schirmer I试验．采用免疫组织化学方法，对兔泪腺组织中炎症因子TGF-β1、IL-1β、TNF—α进行检测。结果C、D、E组SchirmerI试验测量值明显高于B组（P〈0．01），在泪腺导管及腺泡上皮细胞中．IL-1β、TNF—α．阳性表达的细胞数均明显低于B组，而TGF—β1阳性表达的细胞数均明显高于B组（P〈0．01）。以上检测结果C、D组均优于E组（P〈0．05）。结论密蒙花提取物中主要成分为黄酮类物质，可显著抑制雄激素水平降低后兔干眼症的发生。其作用机制可能与黄酮类物质化学结构与雄激素类似，可起到拟雄激素效应，从而调节泪腺局部炎症反应有关。密蒙花提取物可能成为治疗干眼症的新途径。

The effects of buddleja offcinalis extract On dry eye in castrated rabbits

Objective To assess the role of buddleja officinalis extract in basic tear secretion volume, tear film stability, expression of transforming growth factur-β1（TGF-β1）, interleukin-1β（IL-1β）, tumor necrosis factur-α （TNF-α） in the lacrimal gland of castrated rabbits with dry eye, and to discuss the therapeutic effects of buddleja officinabs extract on dry eye caused by a gonadal hormone level imbalanee. Methods Thirty victory rabbits were divided on average into a normal group （A）, model group （B）, a therapy group with single-dose buddleja officinalis extract （C）, a therapy group with double-dose buddleja officinalis extract （D） and a therapy group with genistein （E）. The dry eye model was established with ORX in groups B, C, D and E. Groups C, D and E were gastrically perfused with single-dose or double-dose buddleja officinalis extract or genistein for 30 days. A Schirmer I test （SIT） was used for detection in all rabbits. TGF-β1, IL-1β, and TNF-α were detected with immunohistochemistry. Results The SIT values for groups C, D and E were significantly higher than that for group B （P〈0.01）. The expression of IL-1β, TNF-α in acinar cells and glandular tube cells for groups C, D and E was significantly higher than that for group B （P〈0.01） and the expression of TGF-β1 in acinar cells and glandular tube cells in groups C, D and E was also significantly higher than that of group C and D was positive for group B （P〈0.01）, Any result compared to group E （P〈0.05）. Conclusion The main component of buddleja ofticinalis extract is flavinoid, which displays androgen-like activity, so it can adjust endogenous gonadal hormone level. Buddleja officinalis extract may be an effective therapy for gonadal hormone-associated dry eye because of its characteristics, As a result, it is necessary to do more in-depth research.