| HPLC法测定鼻咽清胶囊黄芩苷的含量 |
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| 引用本文： | 翟保同,高秋华,黄中勋,李钦,王荔.HPLC法测定鼻咽清胶囊黄芩苷的含量[J].河南大学学报(医学版),2004,23(4):43-45. |
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| 作者姓名： | 翟保同 高秋华 黄中勋 李钦 王荔 |
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| 作者单位： | 华中科技大学,湖北,武汉,473009;华中科技大学,化学系,湖北,武汉,473009;河南大学,药学院,河南,开封,475001 |
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| 摘 要： | 目的 :应用RP -HPLC法测定鼻咽清胶囊中黄芩苷的含量。方法 :以甲醇—水—冰醋酸 (4 8∶5 1∶1)为流动相 ,流速为 1.0mL/min ,采用C18化学键合硅胶为固定相 ,在 2 77nm进行测定。结果 :黄芩苷在 0 .2 1μg～ 1.0 5 μg范围内线性关系良好 ,r=0 .9998;平均回收率为 97.9%。结论 :该方法简便、可靠、准确 ,可用于该制剂的质量控制
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| 关 键 词： | RP-HPLC 鼻炎清胶囊 黄芩苷 含量测定 |
| 文章编号： | 1672-7606(2004)04-0043-03 |
| 修稿时间： | 2004年9月25日 |
Determination of baicalin in Biyanning capsules by HPLC |
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| ZHAI Bao-tong ,GAO Qiu-hua ,HUANG Zhong-xun ,LI Qin ,WANG Li.Determination of baicalin in Biyanning capsules by HPLC[J].Journal of Henan University,2004,23(4):43-45. |
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| Authors: | ZHAI Bao-tong GAO Qiu-hua HUANG Zhong-xun LI Qin WANG Li |
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| Institution: | ZHAI Bao-tong 1,GAO Qiu-hua 2,HUANG Zhong-xun 2,LI Qin 3,WANG Li 3 |
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| Abstract: | Objective: To determine the content of baicalin in Biyanning capsules by RP-HPLC.Methods: C18 column was used with the mixture of methanol -H 2O-giacial acetic acid (48∶51∶1) as the mobile phase at a flow rate of 1.0 mL.min -1 with the detection wavelength at 277 nm. Results: The method proved to be linear over the range of 0. 21～1.05 μg, r=0.9998; the recovery of the assay was 97.9%. Conclusion: The method appears to be simple, reliable accurate and can be used to the quality control of the preparation. |
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| Keywords: | RP-HPLC Biyanning capsules baicalin determination |
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